Genome-Wide Identification of the ClpB Gene Family in Tomato and Expression Analysis Under Heat Stress

被引:0
作者
Zhang, Yuemei [1 ]
Yang, Tailai [1 ]
Han, Jiaxi [1 ]
Su, Xiao [1 ]
Cong, Yanqing [1 ]
Zhou, Ming [2 ]
Wang, Yan [1 ]
Lin, Tao [1 ]
机构
[1] China Agr Univ, Coll Hort, Dept Vegetable Sci, Beijing 100193, Peoples R China
[2] Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Key Lab Biol & Genet Improvement Hort Crops North, Minist Agr, Beijing 100097, Peoples R China
基金
中国国家自然科学基金;
关键词
Caseinolytic Protease B; Solanum lycopersicum; high temperature; expression pattern; AAA plus; SHOCK-PROTEIN COMPLEMENTS; AMINO-TERMINAL DOMAIN; ESCHERICHIA-COLI; ACQUIRED THERMOTOLERANCE; BINDING DOMAIN; ATP BINDING; HSP101; TOLERANCE; CHAPERONE; SYSTEM;
D O I
10.3390/ijms252212325
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tomato is a widely grown horticultural crop, and its growth process is often affected by high temperatures. Caseinolytic Protease B (ClpB), a homologous protein to heat shock protein 101 (HSP101), plays a vital role in plant heat adaptation and development. In this study, we identified six SlClpB genes in tomatoes, distributed across four chromosomes. Collinearity analysis revealed that the gene pairs SlClpB-2 and SlClpB-3A, as well as SlClpB-3C and SlClpB-12, resulted from segmental duplication events. Phylogenetic and motif analyses showed that ClpB proteins possess highly conserved domains across different species. We used RNA-seq data to analyze the expression patterns of the ClpB family. Among them, SlClpB-3A and SlClpB-12 exhibited increased expression in multiple tissues under heat stress. Specifically, SlClpB-2, SlClpB-3A, and SlClpB-3C were highly expressed in the fruit orange stage and in flower buds under heat treatment, while in seedlings, SlClpB-2 and SlClpB-3A exhibited heat-induced expression. Real-time quantitative fluorescent PCR (qRT-PCR) results showed that the expression of SlClpB-2 and SlClpB-3A was significantly increased under heat stress in the leaves and buds of Ailsa Craig, Micro-Tom, and M82. Overall, our findings provide valuable insights into the regulatory mechanisms of SlClpB genes in response to heat stress.
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页数:16
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