Application of flow cytometry immunophenotypic analysis for the diagnosis of mature B-cell lymphomas/leukemias

被引：1
作者：
Fang, Hong [1 ]
Wang, Sa A. [1 ]
Medeiros, L. Jeffrey [1 ]
Wang, Wei [1 ]
机构：
[1] Univ Texas MD Anderson Canc Ctr, Dept Hematopathol, Houston, TX USA
来源：
HUMAN PATHOLOGY | 2025年 / 156卷
关键词：
Flow cytometry; B -cell lymphoma; Diagnosis; BONE-MARROW INVOLVEMENT; LIGHT-CHAIN RESTRICTION; MARGINAL ZONE LYMPHOMA; FOLLICULAR LYMPHOMA; DIFFERENTIAL-DIAGNOSIS; LEUKEMIA-VARIANT; EXPRESSION; CD200; LYMPHOCYTOSIS; HYPERPLASIA;
D O I：
10.1016/j.humpath.2024.105711
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Flow cytometry immunophenotyping (FCI) is an important and indispensable tool in the diagnosis of mature Bcell lymphomas/leukemias, particularly for small fine needle aspiration and needle core biopsy specimens which are becoming increasingly popular for diagnostic purposes. FCI has several advantages. Given its multiparametric nature, FCI can analyze the expression of multiple antigens simultaneously on the same cell of interest, qualitatively and quantitively. During the diagnostic process, FCI can provide time sensitive and valuable information for triage of other ancillary studies such as immunohistochemical and molecular studies. In this review, we aim to provide common and practical approaches for using FCI in the diagnostic workup of mature B-cell neoplasms. The immunophenotypic features of common mature B-cell neoplasms as well as diagnostic challenges and pitfalls associated with FCI are also discussed.
引用
收藏
页数：10
相关论文
共 50 条
[21]   Immunophenotypic changes and clinical outcome in B-cell lymphomas treated with rituximab [J].
Seliem, RM ;
Freeman, JK ;
Steingart, RH ;
Hasserjian, RP .
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, 2006, 14 (01) :18-23
[22]   CD200 has an important role in the differential diagnosis of mature B-cell neoplasms by multiparameter flow cytometry [J].
Sandes, Alex F. ;
Chauffaille, Maria de Lourdes ;
Oliveira, Claudia Regina M. C. ;
Maekawa, Yumi ;
Tamashiro, Nair ;
Takao, Tania T. ;
Ritter, Estela C. ;
Rizzatti, Edgar G. .
CYTOMETRY PART B-CLINICAL CYTOMETRY, 2014, 86 (02) :98-105
[23]   A Clinically Applicable Approach to the Classification of B-Cell Non-Hodgkin Lymphomas with Flow Cytometry and Machine Learning [J].
Gaidano, Valentina ;
Tenace, Valerio ;
Santoro, Nathalie ;
Varvello, Silvia ;
Cignetti, Alessandro ;
Prato, Giuseppina ;
Saglio, Giuseppe ;
De Rosa, Giovanni ;
Geuna, Massimo .
CANCERS, 2020, 12 (06) :1-18
[24]   A decision-tree approach for the differential diagnosis of chronic lymphoid leukemias and peripheral B-cell lymphomas [J].
Moraes, L. O. ;
Pedreira, C. E. ;
Barrena, S. ;
Lopez, A. ;
Orfao, A. .
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE, 2019, 178 :85-90
[25]   The Basis and Rational Use of Molecular Genetic Testing in Mature B-cell Lymphomas [J].
Roullet, Michele ;
Bagg, Adam .
ADVANCES IN ANATOMIC PATHOLOGY, 2010, 17 (05) :333-358
[26]   "Double-Hit" Mature B-Cell Lymphomas Show a Common Immunophenotype by Flow Cytometry That Includes Decreased CD20 Expression [J].
Wu, David ;
Wood, Brent L. ;
Dorer, Russell ;
Fromm, Jonathan R. .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2010, 134 (02) :258-265
[27]   Diagnosis and Management of Cutaneous B-Cell Lymphomas [J].
Malachowski, Stephen J. ;
Sun, James ;
Chen, Pei-Ling ;
Seminario-Vidal, Lucia .
DERMATOLOGIC CLINICS, 2019, 37 (04) :443-+
[28]   Flow cytometry is of limited utility in the early identification of "Double-hit" B-cell lymphomas [J].
Platt, Mia Y. ;
DeLelys, Michelle E. ;
Preffer, Frederic I. ;
Sohani, Aliyah R. .
CYTOMETRY PART B-CLINICAL CYTOMETRY, 2013, 84B (03) :143-148
[29]   Diagnostic Algorithm of Common Mature B-Cell Lymphomas by Immunohistochemistry [J].
Wang, Huan-You ;
Zu, Youli .
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, 2017, 141 (09) :1236-1246
[30]   Diagnosis of leptomeningeal disease in diffuse large B-cell lymphomas of the central nervous system by flow cytometry and cytopathology [J].
Schroers, Roland ;
Baraniskin, Alexander ;
Heute, Christoph ;
Vorgerd, Matthias ;
Brunn, Anna ;
Kuhnhenn, Jan ;
Kowoll, Annika ;
Alekseyev, Andriy ;
Schmiegel, Wolff ;
Schlegel, Uwe ;
Deckert, Martina ;
Pels, Hendrik .
EUROPEAN JOURNAL OF HAEMATOLOGY, 2010, 85 (06) :520-528
12345