ASSAY OF PROTEINS IN PRESENCE OF INTERFERING MATERIALS

被引：3431

作者：

BENSADOUN, A

WEINSTEIN, D

机构：

[1] UNIV CALIF, SAN DIEGO, DEPT MED, DIV METAB DIS, LA JOLLA, CA 92037 USA

[2] CORNELL UNIV, DIV NUTR SCI, N205 MARTHA VAN RENSSELAER HALL, ITHACA, NY 14853 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1976年 / 70卷 / 01期

关键词：

D O I：

10.1016/S0003-2697(76)80064-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Lowry protein assay is a sensitive but highly nonspecific procedure. The standard Lowry protein assay was modified, so that protein can be assayed in the presence of interfering chemicals. The method is based on the observation that in the presence of 125 .mu.g/ml of Na-deoxycholate, bovine serum albumin (5-50 .mu.g) is reproducibly precipitated (90-104%) by 6% trichloroacetic acid. Interference by sucrose, glycerol, Tris-HCl, Tricine and EDTA can be eliminated. Protein samples containing carrier ampholytes can also be assayed provided their NaCl concentration is adjusted to 1 M.

引用

页码：241 / 250

页数：10

共 13 条

[1] HEXOSAMINE INTERFERENCE WITH DETERMINATION OF PROTEIN BY LOWRY PROCEDURE [J].

BERG, DH .

ANALYTICAL BIOCHEMISTRY, 1971, 42 (02) :505-&

[2] STUDIES ON THE CONTRACTILE PROTEINS OF MUSCLE .1. THE ATP MYOSIN-B INTERACTION [J].

GELLERT, MF ;

VONHIPPEL, PH ;

SCHACHMAN, HK ;

MORALES, MF .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1959, 81 (06) :1384-1393

[3] INFLUENCE OF SUCROSE ON PROTEIN DETERMINATION BY LOWRY PROCEDURE [J].

GERHARDT, B ;

BEEVERS, H .

ANALYTICAL BIOCHEMISTRY, 1968, 24 (02) :337-&

[4]

HINTON RH, 1969, ANAL BIOCHEM, V24, P337

[5] INTERFERENCE BY DETERGENTS, CHELATING AGENTS, AND BUFFERS WITH LOWRY PROTEIN DETERMINATION [J].

JI, TH .

ANALYTICAL BIOCHEMISTRY, 1973, 52 (02) :517-521

[6]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[7] INTERFERENCE OF SODIUM ETHYLENEDIAMINETETRAACETATE IN DETERMINATION OF PROTEINS AND ITS ELIMINATION [J].

NEURATH, AR .

EXPERIENTIA, 1966, 22 (05) :290-&

[8] INTERFERENCE BY MONOSACCHARIDES WITH ESTIMATION OF TYROSINE AND PROTEINS USING FOLIN-CIOCALTEU PHENOL REAGENT [J].

OSULLIVAN, J ;

MATHISON, GE .

ANALYTICAL BIOCHEMISTRY, 1970, 35 (02) :540-+

[9] EFFECT OF CRYOPROTECTIVE AGENTS ON LOWRY PROTEIN ASSAY [J].

PACE, GW ;

ARCHER, MC ;

TANNENBAUM, SR .

ANALYTICAL BIOCHEMISTRY, 1974, 60 (02) :649-652

[10] INTERFERENCE BY BUFFERS AND OTHER CHEMICALS WITH LOWRY PROTEIN DETERMINATION [J].

PETERS, MA ;

FOUTS, JR .

ANALYTICAL BIOCHEMISTRY, 1969, 30 (02) :299-&

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