Growth Characteristics and Changes in the Active Ingredients of Salviamirzayani iEssential Oilunder Foliar Application of Humic Acid and Amino Acid

Salvia mirzayaniiRech.f. & Esfand. is a medicinal plant from the Lamiaceae family, which has long been of interest to the industry due to its medicinal properties and various metabolites, including terpenoids, phenolic compounds,flavonoids, fatty acids, and sterols. This study was conducted to investigate the effect of foliar spraying of amino acid with concentrations of 0, 0.5, 1, and 1.5 ml/l and humic acid with concentrations of 0, 5, 10, and 15 gr/l along with irrigation water on essential oil compounds and plant growth. The results showed that humic acid (15 ml/l) and amino acid (0.5 ml/l) increased fresh weight, dry weight of leaves, number of inflorescences, and weight of inflorescences, and a further increase in the amino acid level caused a decrease in the measured agricultural characteristics. The use of humic acid 15 mgr/l caused the increase of 5-neo-sedranol, alpha-terpinyl acetate, bicyclogermacrene, and linalyl acetate as the main components of the essential oil to the extent of 37.5, 20.6, 32.5, 94.1 and 22.7% compared to the control respectively. The amount of 5-neo-sedranol and bicyclogermacrone decreased with the increase of amino acid. Amino acid treatment of 1.5 ml/l increased the percentage of linalyl acetate (40.98%), alpha-terpinyl acetate (18.06%), and linalool (10.71%) compared to the control. According to the trend of changes in the percentage of essential oil compounds and the measured agricultural characteristics, it is suggested to use the level of 0.5 mg amino acid and 15 mg of humic acid to reach the most desirable quantitative and qualitative yield of Salvia mirzayanii.