Chromosome-level assembly of the Clinopodium gracile genome

被引:0
作者
Gao, Yubang [1 ]
机构
[1] Nanyang Normal Univ, Sch Life Sci, Nanyang 473061, Henan, Peoples R China
来源
FRONTIERS IN PLANT SCIENCE | 2024年 / 15卷
关键词
genome; chinese herbal medicine; Clinopodium gracile; nanopore sequence; Hi-C assembly; ALIGNMENT; IDENTIFICATION; GENES;
D O I
10.3389/fpls.2024.1489102
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Clinopodium gracile is an important medicinal herb in the Lamiaceae family. This species lacks corresponding genomic resources, which significantly limits the study of its active compound synthesis pathways, breeding practices, and assessment of natural genetic variations. We assembled the chromosomal-level genome of C. gracile using Oxford Nanopore (ONT) technology and Hi-C sequence. The assembled genome is 307.3 Mb in size and consists of 9 chromosomes. The scaffold N50 was 36.3 Mb. The BUSCO completeness (Embryophyta_db10) of the genome was 97.2%. The genome annotates 40,083 protein coding genes. C. gracile and S. miltiorrhiza diverged approximately 30.615 million years ago. C. gracile has not undergone recent species-specific WGD events. A high proportion of young LTRs indicates a recent transposable element (TE) transposition burst in C. gracile.
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页数:6
相关论文
共 35 条
[11]   Recent Advances in Assembly of Complex Plant Genomes [J].
Kong, Weilong ;
Wang, Yibin ;
Zhang, Shengcheng ;
Yu, Jiaxin ;
Zhang, Xingtan .
GENOMICS PROTEOMICS & BIOINFORMATICS, 2023, 21 (03) :427-439
[12]   Transcriptome assembly from long-read RNA-seq alignments with StringTie2 [J].
Kovaka, Sam ;
Zimin, Aleksey, V ;
Pertea, Geo M. ;
Razaghi, Roham ;
Salzberg, Steven L. ;
Pertea, Mihaela .
GENOME BIOLOGY, 2019, 20 (01)
[13]  
Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/nmeth.1923, 10.1038/NMETH.1923]
[14]   Minimap2: pairwise alignment for nucleotide sequences [J].
Li, Heng .
BIOINFORMATICS, 2018, 34 (18) :3094-3100
[15]   OrthoMCL: Identification of ortholog groups for eukaryotic genomes [J].
Li, L ;
Stoeckert, CJ ;
Roos, DS .
GENOME RESEARCH, 2003, 13 (09) :2178-2189
[16]   An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations [J].
MacKenzie, Morgan ;
Argyropoulos, Christos .
MICROMACHINES, 2023, 14 (02)
[17]   A fast, lock-free approach for efficient parallel counting of occurrences of k-mers [J].
Marcais, Guillaume ;
Kingsford, Carl .
BIOINFORMATICS, 2011, 27 (06) :764-770
[18]   CAFE 5 models variation in evolutionary rates among gene families [J].
Mendes, Fabio K. ;
Vanderpool, Dan ;
Fulton, Ben ;
Hahn, Matthew W. .
BIOINFORMATICS, 2020, 36 (22-23) :5516-5518
[19]   Infernal 1.1: 100-fold faster RNA homology searches [J].
Nawrocki, Eric P. ;
Eddy, Sean R. .
BIOINFORMATICS, 2013, 29 (22) :2933-2935
[20]   Benchmarking transposable element annotation methods for creation of a streamlined, comprehensive pipeline [J].
Ou, Shujun ;
Su, Weija ;
Liao, Yi ;
Chougule, Kapeel ;
Agda, Jireh R. A. ;
Hellinga, Adam J. ;
Lugo, Carlos Santiago Blanco ;
Elliott, Tyler A. ;
Ware, Doreen ;
Peterson, Thomas ;
Jiang, Ning ;
Hirsch, Candice N. ;
Hufford, Matthew B. .
GENOME BIOLOGY, 2019, 20 (01)