Integrated Transcriptomic and Metabolomic Analysis Reveals Possible Molecular Mechanisms of Leaf Growth and Development in Disanthus cercidifolius var. longipes

被引:0
|
作者
Tian, Xiaoming [1 ]
Xiang, Guangfeng [1 ]
Mou, Cun [1 ]
Zhu, Lu [1 ]
Song, Jing [2 ]
Li, Gaofei [1 ]
Lv, Hao [1 ]
机构
[1] Hunan Bot Garden, Inst Plant Conservat, Changsha 411006, Peoples R China
[2] Forestry Affairs Ctr Hunan Prov, Changsha 410114, Peoples R China
关键词
<italic>Disanthus cercidifolius</italic> var. <italic>longipes</italic>; photosynthesis; flavonoid synthesis; anthocyanin synthesis; L; LEAVES; ROOTS;
D O I
10.3390/metabo14120654
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Disanthus cercidifolius var. longipes is an ancient relic plant unique to China. However, the typical shade-loving plant is largely exposed to the sun, which poses a major challenge to its conservation. Methods: This study explored dynamic changes in primary and secondary metabolites in D. cercidifolius leaves at different stages of development, combining metabolomics and transcriptome analysis to discuss the differentially accumulated metabolites (DAMs) and differentially expressed genes (DEGs). Results: The DAMs and DEGs were enriched in pathways related to photosynthesis, carbon (C) metabolism, anthocyanin synthesis, plant hormone signal transduction, and flavonoid synthesis. At the initial stage of leaf development, many primary metabolites were synthesized in the leaves. Before leaf maturity, many primary metabolites were converted into secondary metabolites. Combined transcriptome and metabolome analysis showed that the metabolites and genes related to anthocyanin synthesis and flavonoid metabolism were upregulated. In contrast, the genes related to C metabolism and C fixation were downregulated. After leaf maturity, photosynthetic capacity increased, total flavonoid content peaked (implying the strongest photoprotection capacity), and the transformation of anthocyanins and flavonoids was weakened. Conclusions: Light intensity indirectly affects the accumulation of the primary and secondary metabolism of D. cercidifolius. With the enhancement of photoprotection, the photosynthetic energy capacity decreases. It is, therefore, inferable that D. cercidifolius has shading properties and achieves a stable nutrient supply during growth and development through these strategies. Thus, D. cercidifolius protection requires a shaded environment.
引用
收藏
页数:18
相关论文
共 44 条
  • [11] Integrative Transcriptomic and Metabolomic Analysis Reveals Quinoa Leaf Response Mechanisms to Different Phosphorus Concentrations During Filling Stage
    Wang, Hongxin
    Li, Hanxue
    Li, Xiaorong
    Wang, Qianchao
    Liu, Junna
    Zhang, Ping
    Xie, Heng
    Li, Li
    Qin, Peng
    AGRONOMY-BASEL, 2024, 14 (11):
  • [12] Combined full-length transcriptomic and metabolomic analysis reveals the molecular mechanisms underlying nutrients and taste components development in Primulina juliae
    Zhang, Yi
    Yang, Endian
    Liu, Qin
    Zhang, Jie
    Feng, Chen
    BMC GENOMIC DATA, 2024, 25 (01):
  • [13] Integrative Transcriptomic and Metabolomic Analysis Reveals the Molecular Mechanism of Red Maple (Acer rubrum L.) Leaf Coloring
    Luo, Yuanyuan
    Deng, Min
    Zhang, Xia
    Zhang, Damao
    Cai, Wenqi
    Long, Yuelin
    Xiong, Xingyao
    Li, Yanlin
    METABOLITES, 2023, 13 (04)
  • [14] Transcriptomic profiling reveals potential regulatory genes and molecular mechanisms of residual feed intake in Jian carp (Cyprinus carpio var. Jian)
    Wang, Wenqian
    Xu, Yuanfeng
    Zhang, Zhihua
    Jiang, Kemeng
    Li, Jianlin
    Feng, Wenrong
    Sewo, Daniel Yohannes
    Tang, Yongkai
    AQUACULTURE, 2025, 595
  • [15] Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots under Lead Stress
    Guo, Zhaolai
    Yuan, Xinqi
    Li, Ting
    Wang, Sichen
    Yu, Yadong
    Liu, Chang'e
    Duan, Changqun
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (11)
  • [16] Integrated transcriptomic and metabolomic analysis reveals the potential mechanisms underlying indium-induced inhibition of root elongation in wheat plants
    Qian, Ruyi
    Li, Yihao
    Liu, Yuhao
    Sun, Nan
    Liu, Lijuan
    Lin, Xianyong
    Sun, Chengliang
    SCIENCE OF THE TOTAL ENVIRONMENT, 2024, 908
  • [17] Integrated metabolomic and transcriptomic analysis reveals the regulatory mechanisms of flavonoid and alkaloid biosynthesis in the new and old leaves of Murraya tetramera Huang
    Zhou, Tao
    Xing, Qinqin
    Bu, Jiahao
    Han, Wenjun
    Shen, Zhiguo
    BMC PLANT BIOLOGY, 2024, 24 (01):
  • [18] Transcriptomic Analysis Reveals the Molecular Mechanisms of Drought-Stress-Induced Decreases in Camellia sinensis Leaf Quality
    Wang, Weidong
    Xin, Huahong
    Wang, Mingle
    Ma, Qingping
    Wang, Le
    Kaleri, Najeeb A.
    Wang, Yuhua
    Li, Xinghui
    FRONTIERS IN PLANT SCIENCE, 2016, 7
  • [19] Integrated transcriptomic and metabolomic analysis reveals the molecular basis of tissue-specific accumulation of bioactive steroidal alkaloids in Fritillaria unibracteata
    Liao, Hai
    Quan, Huige
    Huang, Binhan
    Ji, Huiyue
    Zhang, Tian
    Chen, Jiao
    Zhou, Jiayu
    PHYTOCHEMISTRY, 2023, 214
  • [20] Integrated Transcriptomic and Metabolomic Analysis Reveals the Mechanism of Gibberellic Acid Regulates the Growth and Flavonoid Synthesis in Phellodendron chinense Schneid Seedlings
    Yang, Lv
    Luo, Shengwei
    Jiao, Jing
    Yan, Wende
    Zeng, Baiquan
    He, Hanjie
    He, Gongxiu
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (22)