Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel

被引：0

作者：

Lemaitre, Pierre ^{[1
]}

Mathieu, Chantal ^{[1
]}

Gysemans, Conny ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Clin & Expt Endocrinol CEE, CHROMETA, O&N1bis, Campus Gasthuisberg, Leuven, Belgium

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 04期

关键词：

ORIGIN;

D O I：

10.1016/j.xpro.2024.103492

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The innate and adaptive immune systems, though often studied separately, interact deeply and respond to stimuli simultaneously, with leukocytes displaying a range of proto anti-inflammatory phenotypes. This protocol details a procedure for characterizing murine innate and adaptive immune phenotypes using a 40-color full-spectral flow cytometry panel. We describe steps for organ collection, sample preparation, immunofluorescent staining, and acquisition to reproducibly and cost-effectively study tissue-resident leukocytes, their subpopulations, and inflammatory status in various organs.

引用

页数：18