Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel

被引：0
|
作者：
Lemaitre, Pierre [1 ]
Mathieu, Chantal [1 ]
Gysemans, Conny [1 ]
机构：
[1] Katholieke Univ Leuven, Clin & Expt Endocrinol CEE, CHROMETA, O&N1bis, Campus Gasthuisberg, Leuven, Belgium
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 04期
关键词：
ORIGIN;
D O I：
10.1016/j.xpro.2024.103492
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The innate and adaptive immune systems, though often studied separately, interact deeply and respond to stimuli simultaneously, with leukocytes displaying a range of proto anti-inflammatory phenotypes. This protocol details a procedure for characterizing murine innate and adaptive immune phenotypes using a 40-color full-spectral flow cytometry panel. We describe steps for organ collection, sample preparation, immunofluorescent staining, and acquisition to reproducibly and cost-effectively study tissue-resident leukocytes, their subpopulations, and inflammatory status in various organs.
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页数：18
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