Line-Shifting Triarylmethyl Radicals for Imaging of Enzyme Activity Using Overhauser-Enhanced Magnetic Resonance Imaging: Application to Alkaline Phosphatase

被引：1
|
作者：
Velayutham, Murugesan [1 ,2 ]
Poncelet, Martin [2 ,3 ]
Enomoto, Ayano [4 ]
Huffman, Justin L. [2 ,3 ]
Pandya, Virat G. [2 ,3 ]
Ichikawa, Kazuhiro [4 ]
Khramtsov, Valery V. [1 ,2 ]
Driesschaert, Benoit [2 ,3 ,5 ]
机构：
[1] West Virginia Univ, Sch Med, Dept Biochem & Mol Med, Morgantown, WV 26506 USA
[2] West Virginia Univ, Vivo Multifunct Magnet Resonance Ctr, Robert C Byrd Hlth Sci Ctr, Morgantown, WV 26506 USA
[3] West Virginia Univ, Sch Pharm, Dept Pharmaceut Sci, Morgantown, WV 26506 USA
[4] Nagasaki Int Univ, Fac Pharmaceut Sci, Nagasaki 8593298, Japan
[5] West Virginia Univ, C Eugene Bennett Dept Chem, Morgantown, WV 26506 USA
来源：
CHEMICAL & BIOMEDICAL IMAGING | 2024年
关键词：
EPR; TAM radical; enzyme activity; alkaline phosphatase; OMRI; ELECTRON-PARAMAGNETIC-RESONANCE; LARGE-SCALE SYNTHESIS; IN-VIVO; TRIS(2,3,5,6-TETRATHIAARYL)METHYL CATIONS; EPR; OXYGEN; NITROXIDES; ACIDS; PH;
D O I：
10.1021/cbmi.4c00059
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Enzyme catalytic activities are critical biomarkers of tissue states under physiological and pathophysiological conditions. However, the direct measurement and imaging of enzyme activity in vivo remains extremely challenging. We report the synthesis and characterization of the first stable triarylmethyl (TAM) radical substrate of alkaline phosphatase (TAM-ALPs). The enzymatic dephosphorylation of TAM-ALPs results in a drastic change in its electron paramagnetic resonance (EPR) spectrum that can be used to image enzyme activity using EPR-based technologies. TAM-ALPs and their enzyme products were fully characterized using EPR and HPLC-MS techniques. A proof of concept of imaging enzyme activity using Overhauser-enhanced magnetic resonance imaging was demonstrated in vitro. This study clearly demonstrates the potential of EPR-based imaging technologies associated with TAM spin probes to map enzyme activity in vivo in future studies.
引用
收藏
页数：10
相关论文
共 39 条
  • [31] Dictionary learning LASSO for feature selection with application to hepatocellular carcinoma grading using contrast enhanced magnetic resonance imaging
    Lei, Lei
    Du, Li-Xin
    He, Ying-Long
    Yuan, Jian-Peng
    Wang, Pan
    Ye, Bao-Lin
    Wang, Cong
    Hou, ZuJun
    FRONTIERS IN ONCOLOGY, 2023, 13
  • [32] USING GADOLINIUM-ENHANCED MAGNETIC-RESONANCE-IMAGING LESIONS TO MONITOR DISEASE-ACTIVITY IN MULTIPLE-SCLEROSIS
    MCFARLAND, HF
    FRANK, JA
    ALBERT, PS
    SMITH, ME
    MARTIN, R
    HARRIS, JO
    PATRONAS, N
    MALONI, H
    MCFARLIN, DE
    ANNALS OF NEUROLOGY, 1992, 32 (06) : 758 - 766
  • [33] Using "On/Off" 19F NMR/Magnetic Resonance Imaging Signals to Sense Tyrosine Kinase/Phosphatase Activity in Vitro and in Cell Lysates
    Zheng, Zhen
    Sun, Hongbin
    Hu, Chen
    Li, Gongyu
    Liu, Xiaomei
    Chen, Peiyao
    Cui, Yusi
    Liu, Jing
    Wang, Junfeng
    Liang, Gaolin
    ANALYTICAL CHEMISTRY, 2016, 88 (06) : 3363 - 3368
  • [34] In vivo evaluation of cellular activity in alpha CaMKII heterozygous knockout mice using manganese-enhanced magnetic resonance imaging (MEMRI)
    Hattori, Satoko
    Hagihara, Hideo
    Ohira, Koji
    Aoki, Ichio
    Saga, Tsuneo
    Suhara, Tetsuya
    Higuchi, Makoto
    Miyakawa, Tsuyoshi
    FRONTIERS IN INTEGRATIVE NEUROSCIENCE, 2013, 7
  • [35] The ATHEROMA (Atorvastatin Therapy: Effects on Reduction of Macrophage Activity) Study Evaluation Using Ultrasmall Superparamagnetic Iron Oxide-Enhanced Magnetic Resonance Imaging in Carotid Disease
    Tang, Tjun Y.
    Howarth, Simon P. S.
    Miller, Sam R.
    Graves, Martin J.
    Patterson, Andrew J.
    U-King-Im, Jean-Marie
    Li, Zhi Y.
    Walsh, Stewart R.
    Brown, Andrew P.
    Kirkpatrick, Peter J.
    Warburton, Elizabeth A.
    Hayes, Paul D.
    Varty, Kevin
    Boyle, Jonathan R.
    Gaunt, Michael E.
    Zalewski, Andrew
    Gillard, Jonathan H.
    JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2009, 53 (22) : 2039 - 2050
  • [36] ASSESSMENT OF DISEASE ACTIVITY USING CONTRAST-ENHANCED MAGNETIC RESONANCE IMAGING IN CHILDREN AND YOUNG PEOPLE WITH SUSPECTED HIP ARTHRITIS-CAN WE DO BETTER?
    Young, Francesca M.
    Broadley, Penny
    Lang, Isla
    Offiah, Amaka C.
    McMahon, Anne-Marie
    Hawley, Daniel P.
    RHEUMATOLOGY, 2016, 55 : 58 - 58
  • [37] Delayed Contrast Enhanced Magnetic Resonance Simulation Imaging in Prone Position for Radiotherapy After Breast Conserving Surgery: Assessing the Application in Lumpectomy Cavity Delineation Using Deformable Image Registration
    Zhao, C.
    Li, J.
    Wang, W.
    INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 2020, 108 (03): : E331 - E332
  • [38] Feasibility of High-Resolution 3-Dimensional Sampling Perfection with Application-Optimized Contrast Using Different Flip Angle Evolution Imaging for the Preoperative Detection of Parasinus Meningioma: A Pilot Study Compared with Contrast-Enhanced Magnetic Resonance Venography
    Yan Tianfang
    Xu Liang
    Wei Yukui
    Lin Qingtang
    Jiao Liqun
    Xu Geng
    WORLD NEUROSURGERY, 2018, 119 : E623 - E630
  • [39] A Novel Approach for Imaging of Thoracic Outlet Syndrome Using Contrast-Enhanced Magnetic Resonance Angiography (CE-MRA), Short Inversion Time Inversion Recovery Sampling Perfection with Application-Optimized Contrasts Using Different Flip Angle Evolutions (T2-STIR-SPACE), and Volumetric Interpolated Breath-Hold Examination (VIBE)
    Zhang, Tonghua
    Xu, Zhengdao
    Chen, Jianxin
    Liu, Zongbao
    Wang, Tao
    Hu, Yijiang
    Shen, Liping
    Xue, Feifei
    MEDICAL SCIENCE MONITOR, 2019, 25 : 7617 - 7623
1234