Relevance of Circulating microRNA, and their Association with Islet Cell Autoantibodies in Type 1 Diabetes Pathogenesis

被引:0
作者
Santos, Aritania S. [1 ]
Santos-Bezerra, Daniele Pereira [1 ]
Ferreira, Ludmila Rodrigues Pinto [2 ]
Bando, Silvia Y. [3 ]
Alves, Lais Isidoro [1 ]
Cunha-Neto, Edecio [4 ]
da Silva, Maria Elizabeth Rossi [1 ,5 ]
机构
[1] Univ Sao Paulo, Fac Med, Lab Carboidratos & Radioimunoensaios, Ave Dr Arnaldo 455, BR-01246903 Sao Paulo, Brazil
[2] Univ Fed Minas Gerais, Inst Biol Sci, Dept Morphol, RNA Syst Biol Lab, Belo Horizonte, MG, Brazil
[3] Univ Sao Paulo, Fac Med, Dept Pediat, Sao Paulo, Brazil
[4] Univ Sao Paulo, Heart Inst, Inst Invest Immunol INCT 3, Sch Med,Lab Immunol, Sao Paulo, Brazil
[5] Univ Sao Paulo, Hosp Clin, Fac Med, Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
Microrna; Type; 1; diabetes; Pathogenesis; Autoantibodies; EXPRESSION;
D O I
10.1016/j.arcmed.2024.103114
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Background. Aims/hypothesis. The role of microRNAs (miRNAs) in the pathogenesis and progression of type 1 diabetes (T1D) has been described, but data remain scarce and conflicting. Objectives. To evaluate the potential biological involvement of miRNA expression in the immune response and beta cell function in T1D. Methods. We screened 10 serum miRNAs from 142 subjects divided into three groups: healthy individuals (control group; n = 52) and patients at different stages of T1D progression, from the initial immunological manifestation, presenting islet cell autoantibodies (AbP group; n = 39), to partial and severe beta cell damage in T1D (recent T1D group; n = 51). Results. Three miRNAs (miR-200c-3p, miR-301a-3p, and miR-382-5p) were highly expressed in the AbP and/or recent T1D groups compared to the control group. Furthermore, in the AbP group, miR-301a-3p and miR-382-5p were positively correlated with insulin autoantibody levels and miR-382-5p was negatively correlated with C-peptide levels. In the recent T1D group, miR-200c-3p expression was positively correlated with IA-2A levels. Enrichment analysis of differentially expressed miRNAs showed their involvement in immune response, inflammatory pathways, proliferation/survival/apoptosis mechanisms, bacterial and viral infection, and insulin resistance. Conclusion. Our data indicated that miR-200c-3p, miR-301a-3p, and miR-382-5p might be involved in T1D pathogenesis. Proliferative, metabolic, and immune responses were main pathways associated with serum miRNA target genes. (c) 2024 Published by Elsevier Inc. on behalf of Instituto Mexicano del Seguro Social (IMSS).
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页数:11
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