Genetically encoded bioluminescent glucose indicator for biological research

被引：0

作者：

Tanaka, Rikuto ^{[1
]}

Sugiura, Kazunori ^{[2
]}

Osabe, Kenji ^{[2
]}

Hattori, Mitsuru ^{[2
]}

Nagai, Takeharu ^{[1
,2
,3
]}

机构：

[1] Univ Osaka, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan

[2] Univ Osaka, SANKEN, Osaka, Ibaraki 5670047, Japan

[3] Hokkaido Univ, Res Inst Elect Sci, Sapporo, Hokkaido 0010021, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2025年 / 742卷

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

Glucose metabolism; Genetically encoded bioluminescent indicator; Bioluminescence imaging; FLUORESCENT PROTEIN; SENSITIVITY; GALACTOSE; CELLS; MODEL;

D O I：

10.1016/j.bbrc.2024.151092

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucose is an essential energy source in living cells and is involved in various phenomena. To understand the roles of glucose, measuring cellular glucose levels is important. Here, we developed a bioluminescent glucose indicator called LOTUS-Glc. Unlike fluorescence, bioluminescence doesn't require excitation light when imaging. Using LOTUS-Glc, we demonstrated drug effect evaluation, concurrent use with the optogenetic tool in HEK293T cells, and the measurement of light-dependent glucose fluctuations in plant-derived protoplasts. LOTUS-Glc would be a useful tool for understanding the roles of glucose in living organisms.

引用

页数：6

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