The Influence of Sodium Humate on the Biosynthesis and Contents of Flavonoid Constituents in Lemons

被引:0
|
作者
Xu, Nianao [1 ]
Yang, Fan [2 ]
Dai, Weifeng [1 ]
Yuan, Cheng [1 ]
Li, Jinxue [2 ]
Zhang, Hanqi [1 ]
Ren, Youdi [1 ]
Zhang, Mi [1 ]
机构
[1] Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Peoples R China
[2] Yunnan Acad Agr Sci, Inst Trop & Subtrop Cash Crops, Ruili 678600, Peoples R China
来源
PLANTS-BASEL | 2024年 / 13卷 / 20期
关键词
sodium humate; flavonoid; action mechanism; lemon; HCT; F5H; phenylalanine pathway; humic acid; SECONDARY METABOLITES; TRANSPORT;
D O I
10.3390/plants13202888
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Sodium humate (SH) is the sodium salt of humic acid. Our previous research has demonstrated that SH has the ability to enhance the levels of total flavonoids in various parts of lemons, including the leaves, peels, pulps, and seeds, thereby improving the quality of lemons. In the current study, the regulation effect of SH on the biosynthesis and content of lemon flavonoid compounds was examined using transcriptome sequencing technology and flavonoid metabolomic analysis. Following SH treatment, the transcriptome sequencing analysis revealed 320 differentially expressed genes (DEGs) between samples treated with SH and control (CK) samples, some of which were associated with the phenylalanine pathway by KEGG annotation analysis. The levels of seven flavonoid compounds identified in lemon peels were observed to increase, and eriocitrin and isoorientin were identified as differential metabolites (DMs, VIP > 1) using OPLS-DA analysis. The integrated analysis of transcriptomics and flavonoid metabolomics indicates that SH treatment induces alterations in gene expression and metabolite levels related to flavonoid synthesis. Specifically, SH influences flavonoid biosynthesis by modulating the activity of key enzymes in the phenylalanine pathway, including HCT (O-hydroxycinnamoyltransferase) and F5H (ferulate-5-hydroxylase).
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页数:13
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