Mitigating ethyl carbamate production in Chinese rice wine: Role of raspberry extract

被引：0
作者：
Liu, Yuxin [1 ]
Shen, Chi [1 ]
Wang, Xiaoyu [1 ]
Xiao, Chaogeng [2 ]
Luo, Zisheng [3 ]
Sun, Guochang [4 ]
Lu, Wenjing [2 ]
Tian, Rungang [1 ]
Dong, Lijia [1 ]
Han, Xueyuan [1 ,3 ]
机构：
[1] Shaoxing Univ, Sch Life & Environm Sci, Shaoxing 312000, Peoples R China
[2] Zhejiang Acad Agr Sci, Inst Food Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
[3] Zhejiang Univ, Coll Biosyst Engn & Food Sci, Zhejiang Key Lab Agrofood Proc, Key Lab Agroprod Postharvest Handling,Minist Agr &, Hangzhou 310058, Peoples R China
[4] Zhejiang Kuaijishan Shaoxing Rice Wine Co Ltd, Shaoxing 312030, Peoples R China
来源：
JOURNAL OF INTEGRATIVE AGRICULTURE | 2025年 / 24卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Chinese rice wine; ethyl carbamate; raspberry extract; Saccharomyces cerevisiae; arginine metabolism; LACTIC-ACID BACTERIA; SACCHAROMYCES-CEREVISIAE; ARGININE DEIMINASE; ALCOHOLIC FERMENTATION; ARGINASE ACTIVITY; AMINO-ACIDS; METABOLISM; UREA; CHROMATOGRAPHY; EXPRESSION;
D O I：
10.1016/j.jia.2024.08.021
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
This study investigated the use of raspberry extract (RBE) for mitigating ethyl carbamate (EC) accumulation in Chinese rice wine (Huangjiu), a traditional fermented beverage. It focused on the addition of RBE to the fermentation mash and its effects on EC levels. The results showed a significant reduction in EC production that could be attributed to RBE's role in altering urea and citrulline catabolism and inhibiting arginine metabolism, thus preventing EC precursors from reacting with ethanol. Additionally, RBE enhanced the rice wine's flavor profile, as shown by volatile component and amino acid analysis. This study also explored RBE's impact on the metabolism of arginine by Saccharomyces cerevisiae in a simulated fermentation environment, and found increased arginine, reduced urea and citrulline levels, altered enzyme activities, and gene expression changes in the arginine metabolism and transport pathways. In conclusion, the results clearly demonstrated RBE's efficacy in reducing the EC content in Chinese rice wine, offering valuable insights for EC reduction strategies.
引用
收藏
页码：353 / 365
页数：13
相关论文
共 58 条
  • [1] Alberto M.R., Manca de Nadra M.C., Arena M.E., Influence of phenolic compounds on the growth and arginine deiminase system in a wine lactic acid bacterium, Brazilian Journal of Microbiology, 43, pp. 167-176, (2012)
  • [2] Benucci I., Esti M., Arginase activity characterization during alcoholic fermentation by sequential inoculation with non-Saccharomyces and Saccharomyces yeast, Food and Bioprocess Technology, 14, pp. 1996-2003, (2021)
  • [3] Benucci I., Fiorelli V., Lombardelli C., Liburdi K., Esti M., Kinetic characterization of arginase from Saccharomyces cerevisiae during alcoholic fermentation at different temperatures, LWT-Food Science and Technology, 82, pp. 268-273, (2017)
  • [4] Cao Y., Xie G.F., Wu C., Lu J., A study on characteristic flavor compounds in traditional Chinese rice wine - Guyue Longshan rice wine, Journal of the Institute of Brewing, 116, pp. 182-189, (2010)
  • [5] Carrasco P., Perez-Ortin J.E., li del Olmo M., Arginase activity is a useful marker of nitrogen limitation during alcoholic fermentations, Systematic and Applied Microbiology, 26, pp. 471-479, (2003)
  • [6] Chen D.W., Ren Y.P., Zhong Q.D., Shao Y., Zhao Y.F., Wu Y.N., Ethyl carbamate in alcoholic beverages from China: Levels, dietary intake, and risk assessment, Food Control, 72, pp. 283-288, (2017)
  • [7] Chen Z., Jiang J.Y., Li X.B., Xie Y.W., Jin Z.X., Wang X.Y., Li Y.L., Zhong Y.J., Lin J.J., Yang W.Q., Bioactive compounds and fruit quality of Chinese raspberry, Rubus chingii Hu varied with genotype and phenological phase, Scientia Horticulturae, 281, (2021)
  • [8] Crepin L., Truong N.M., Bloem A., Sanchez I., Dequin S., Camarasa C., Management of multiple nitrogen sources during wine fermentation by Saccharomyces cerevisiae, Applied and Environmental Microbiology, 83, (2017)
  • [9] Dahabieh M.S., Metabolic engineering of industrial yeast strains to minimize the production of ethyl carbamate in grape and sake wine, (2008)
  • [10] Deshavath N.N., Mukherjee G., Goud V.V., Veeranki V.D., Sastri C.V., Pitfalls in the 3,5-dinitrosalicylic acid (DNS) assay for the reducing sugars: Interference of furfural and 5-hydroxymethylfurfural, International Journal of Biological Macromolecules, 156, pp. 180-185, (2020)
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