Transcriptomics and physiological indicators analyzed the delayed effect of carboxymethyl chitosan on lignification of okra during postharvest cold storage

被引：1

作者：

Wei, Lijuan ^{[1
]}

Gao, Shunjiao ^{[1
]}

Li, Fan ^{[1
]}

Zhang, Xinyue ^{[1
]}

Peng, Yue ^{[1
]}

Liu, Yaowen ^{[1
]}

Zhang, Yiqin ^{[1
]}

Qin, Wen ^{[1
]}

机构：

[1] Sichuan Agr Univ, Coll Food Sci, Yaan 625014, Peoples R China

来源：

POSTHARVEST BIOLOGY AND TECHNOLOGY | 2025年 / 222卷

关键词：

Polysaccharide preservation; Lignin; Abelmoschus esculentus; Transcription expression; Cell wall components; ABELMOSCHUS-ESCULENTUS L; ANTIOXIDANT PROPERTIES; LIGNIN ACCUMULATION; ENZYME-ACTIVITY; DERIVATIVES; EXPRESSION; FRUIT;

D O I：

10.1016/j.postharvbio.2025.113394

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Lignification is one of the key factors leading to postharvest quality deterioration of fruit and vegetables. In this study, the effects of carboxymethyl chitosan (CMCS) on nutritional characteristics, cell wall substances, key enzyme activities, transcriptome and related gene expression levels of okra during postharvest low temperature storage were investigated. The results showed that CMCS could maintain the nutritional quality of postharvest okra, delay the accumulation of rigid components (lignin, cellulose, hemicellulose and pectin) in cell wall, and inhibit the enzyme activities of phenylpropanoid pathway (PAL, 4CL, CAD and POD). Transcriptome data analysis and qRT-PCR results showed that CMCS down-regulated the expression levels of lignin synthesis-related genes ( AePAL, AeC4H, Ae4CL, AeCAD, AeCOMT, AePOD). MYB is an important transcription factor (TF) regulating lignin accumulation. The results of gene promoter analysis in qRT-PCR showed that 14 genes contained multiple MYB cis-acting elements. Compared with CK, CMCS inhibited the expression of multiple upstream AeMYBs. Collectively, CMCS is a promising green preservative by inhibiting the metabolism of nutrients, the enzyme activities of the phenylpropanoid pathway, and the expression levels of related genes and TFs, thereby delaying the accumulation of lignin during postharvest cold storage of okra and maintaining postharvest quality.

引用

页数：15

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