Prevention of Sunlight-Induced Cell Damage by Selective Blue-Violet-Light-Filtering Lenses in A2E-Loaded Retinal Pigment Epithelial Cells

被引:0
作者
Barrau, Coralie [1 ]
Marie, Melanie [2 ]
Ehrismann, Camille [1 ]
Gondouin, Pauline [2 ]
Sahel, Jose-Alain [2 ,3 ,4 ]
Villette, Thierry [1 ]
Picaud, Serge [2 ]
机构
[1] R&D Essilor Int, 147 Rue Paris, F-94220 Charenton Le Pont, France
[2] Sorbonne Univ, CNRS, INSERM, Inst Vis, F-75012 Paris, France
[3] Quinze Vingts Natl Ophthalmol Hosp, INSERM, DGOS Clin Invest Ctr 1423, 28 Rue Charenton, F-75012 Paris, France
[4] Univ Pittsburgh, Sch Med & Med Ctr, Dept Ophthalmol, Pittsburgh, PA 15213 USA
关键词
blue-violet light; oxidative stress; photoprotection; filter; MACULAR DEGENERATION; INTRAOCULAR LENSES; RISK-FACTOR; LIPOFUSCIN; EXPOSURE; DEATH; GRP78; A2-E;
D O I
10.3390/antiox13101195
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Blue light accelerates retinal aging. Previous studies have indicated that wavelengths between 400 and 455 nm are most harmful to aging retinal pigment epithelia (RPE). This study explored whether filtering these wavelengths can protect cells exposed to broad sunlight. Primary porcine RPE cells loaded with 20 mu M A2E were exposed to emulated sunlight filtered through eye media at 1.8 mW/cm2 for 18 h. Filters selectively filtering out light over 400-455 nm and a dark-yellow filter were interposed. Cell damage was measured by apoptosis, hydrogen peroxide (H2O2) production, and mitochondrial membrane potential (MMP). Sunlight exposure increased apoptosis by 2.7-fold and H2O2 by 4.8-fold, and halved MMP compared to darkness. Eye Protect SystemTM (EPS) technology, filtering out 25% of wavelengths over 400-455 nm, reduced apoptosis by 44% and H2O2 by 29%. The Multilayer Optical Film (MOF), at 80% of light filtered, reduced apoptosis by 91% and H2O2 by 69%, and increased MMP by 73%, overpassing the dark-yellow filter. Photoprotection increased almost linearly with blue-violet light filtering (400-455 nm) but not with total blue filtering (400-500 nm). Selective filters filtering out 25% (EPS) to 80% (MOF) of blue-violet light offer substantial protection without affecting perception or non-visual functions, making them promising for preventing light-induced retinal damage with aesthetic acceptance for permanent wear.
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页数:16
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