Green UV-spectrophotometric method for estimation of esomeprazole in injection dosage

A new spectroscopic method had been proposed to determine esomeprazole in pure form and pharmaceutical preparation. The method is based on the hydrolysis of esomeprazole by adding hydrochloric acid to produce a product that gives the highest absorption at a wavelength of 352 nm. In comparison, the solution of esomeprazole alone gave the highest absorption at 300 nm, indicating the suggested method gave a wave displacement. The resulting solution was soluble in water and had a molar absorptivity coefficient of 7.7 x 103 l/mol. cm. The range of estimate obeying Beer's law was from 1-40 mu g/mL with a determination coefficient (R2) of 0.9997. Some of the analytical constants related to the method of estimation were calculated, including stability, where the result was stable for an hour, and the Sandall sensitivity value was calculated and mentioned, 0.044 mu g/cm2. The method was also applied to estimate the compound under study in pharmaceutical preparation (tablet), and reliable analytical results were obtained. The greenness of the suggested methods was evaluated, demonstrating the minimum hazardous effect on the environment.