Identification of Rv1133c (MetE) as a marker of Mycobacterium tuberculosis replication and as a highly immunogenic antigen with potential immunodiagnostic power
被引:0
|
作者:
Iacobino, Angelo
论文数: 0引用数: 0
h-index: 0
机构:
Ist Super Sanita, Dipartimento Malattie Infett, Rome, ItalyIst Super Sanita, Dipartimento Malattie Infett, Rome, Italy
Iacobino, Angelo
[1
]
Teloni, Raffaela
论文数: 0引用数: 0
h-index: 0
机构:
Ist Super Sanita, Dipartimento Malattie Infett, Rome, ItalyIst Super Sanita, Dipartimento Malattie Infett, Rome, Italy
Teloni, Raffaela
[1
]
Mancone, Carmine
论文数: 0引用数: 0
h-index: 0
机构:
Sapienza Univ Roma, Dipartimento Med Mol, Rome, ItalyIst Super Sanita, Dipartimento Malattie Infett, Rome, Italy
Mancone, Carmine
[2
]
Facchiano, Francesco
论文数: 0引用数: 0
h-index: 0
机构:
Ist Super Sanita, Dipartimento Oncol & Med Mol, Rome, ItalyIst Super Sanita, Dipartimento Malattie Infett, Rome, Italy
Facchiano, Francesco
[3
]
Di Giamberardino, Alessandra
论文数: 0引用数: 0
h-index: 0
机构:
Sapienza Univ Roma, Dipartimento Med Mol, Rome, ItalyIst Super Sanita, Dipartimento Malattie Infett, Rome, Italy
Di Giamberardino, Alessandra
[2
]
Senatore, Cinzia
论文数: 0引用数: 0
h-index: 0
机构:
Ist Super Sanita, Dipartimento Oncol & Med Mol, Rome, ItalyIst Super Sanita, Dipartimento Malattie Infett, Rome, Italy
Senatore, Cinzia
[3
]
Di Virgilio, Antonio
论文数: 0引用数: 0
h-index: 0
机构:
Ist Super Sanita, Ctr Sperimentaz Benessere Anim, Rome, ItalyIst Super Sanita, Dipartimento Malattie Infett, Rome, Italy
Di Virgilio, Antonio
[4
]
Lanni, Alessio
论文数: 0引用数: 0
h-index: 0
机构:
Ist Super Sanita, Dipartimento Malattie Infett, Rome, ItalyIst Super Sanita, Dipartimento Malattie Infett, Rome, Italy
Lanni, Alessio
[1
]
Giannoni, Federico
论文数: 0引用数: 0
h-index: 0
机构:
Ist Super Sanita, Dipartimento Malattie Infett, Rome, ItalyIst Super Sanita, Dipartimento Malattie Infett, Rome, Italy
Giannoni, Federico
[1
]
Nisini, Roberto
论文数: 0引用数: 0
h-index: 0
机构:
Ist Super Sanita, Dipartimento Malattie Infett, Rome, ItalyIst Super Sanita, Dipartimento Malattie Infett, Rome, Italy
Nisini, Roberto
[1
]
Mariotti, Sabrina
论文数: 0引用数: 0
h-index: 0
机构:
Ist Super Sanita, Dipartimento Malattie Infett, Rome, ItalyIst Super Sanita, Dipartimento Malattie Infett, Rome, Italy
Mariotti, Sabrina
[1
]
机构:
[1] Ist Super Sanita, Dipartimento Malattie Infett, Rome, Italy
[2] Sapienza Univ Roma, Dipartimento Med Mol, Rome, Italy
[3] Ist Super Sanita, Dipartimento Oncol & Med Mol, Rome, Italy
[4] Ist Super Sanita, Ctr Sperimentaz Benessere Anim, Rome, Italy
The immunization of mice with the sterile culture medium supernatants of Mycobacterium tuberculosis (Mtb) H37Rv permitted the production of several monoclonal antibodies (mAbs) specific for secreted and/or released antigens. Two mAbs bound and immunoprecipitated an 80-kDa protein that was identified by mass spectrometry as Rv1133c, the methionine synthase MetE. The protein MetE is ubiquitous among prokaryota and shows a significant sequence homology in many bacteria. We produced both the full-length recombinant MetE and its N-terminal fragment, whose sequence is more conserved among mycobacteria, to select mAbs recognizing an Mtb-specific region of MetE. Finally, we produced and selected eight mAbs that specifically detect the MetE protein in the supernatant and cell lysate of Mtb and BCG, but not other bacteria such as non-tuberculous mycobacteria (NTM), Streptococcus pneumoniae, Staphylococcus aureus, Acinetobacter baumanii, or Escherichia coli. Taking advantage of our mAbs, we studied (i) the vitamin B12 dependence for the synthesis of MetE in Mtb and NTM and (ii) the kinetics of MetE production and secretion in supernatants during the in vitro reproduced replicative, dormant, and resuscitation cycle of Mtb. Our data demonstrate that dormant Mtb, which are assumed to be prevalent in latent infections, as well as NTM do not produce and secrete MetE. Results indicate an unexpected specificity for Mtb of our anti-MetE mAbs and encourage the use of rMetE and our mAbs as tools for the immunodiagnosis of TB and its stages.
机构:
Sungkyunkwan Univ, Sch Med, Div Pulm & Crit Care Med, Dept Med,Samsung Med Ctr, Seoul, South KoreaChungnam Natl Univ, Coll Med, Dept Microbiol, Taejon, South Korea
Kim, S. -Y.
Shin, A. -R.
论文数: 0引用数: 0
h-index: 0
机构:
Chungnam Natl Univ, Coll Med, Dept Microbiol, Taejon, South Korea
Chungnam Natl Univ, Infect Signaling Network Res Ctr, Coll Med, Taejon, South KoreaChungnam Natl Univ, Coll Med, Dept Microbiol, Taejon, South Korea
Shin, A. -R.
Kim, H. -J.
论文数: 0引用数: 0
h-index: 0
机构:
Chungnam Natl Univ, Coll Med, Dept Microbiol, Taejon, South KoreaChungnam Natl Univ, Coll Med, Dept Microbiol, Taejon, South Korea
Kim, H. -J.
Cho, S. -N.
论文数: 0引用数: 0
h-index: 0
机构:
Yonsei Univ, Coll Med, Dept Microbiol, Seoul, South Korea
Yonsei Univ, Coll Med, Brain Korea Project Med Sci 21, Seoul, South KoreaChungnam Natl Univ, Coll Med, Dept Microbiol, Taejon, South Korea
Cho, S. -N.
Park, J. -K.
论文数: 0引用数: 0
h-index: 0
机构:
Chungnam Natl Univ, Coll Med, Dept Microbiol, Taejon, South Korea
Chungnam Natl Univ, Coll Med, Canc Res Inst, Taejon, South KoreaChungnam Natl Univ, Coll Med, Dept Microbiol, Taejon, South Korea
Park, J. -K.
Shin, S. J.
论文数: 0引用数: 0
h-index: 0
机构:
Chungnam Natl Univ, Coll Med, Dept Microbiol, Taejon, South Korea
Chungnam Natl Univ, Infect Signaling Network Res Ctr, Coll Med, Taejon, South KoreaChungnam Natl Univ, Coll Med, Dept Microbiol, Taejon, South Korea
机构:
Chungnam Natl Univ, Dept Microbiol, Taejon 301747, South Korea
Chungnam Natl Univ, Res Inst Med Sci, Taejon 301747, South KoreaChungnam Natl Univ, Dept Microbiol, Taejon 301747, South Korea
Shin, S. J.
Kim, S. -Y.
论文数: 0引用数: 0
h-index: 0
机构:
Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Med,Div Pulm & Crit Care Med, Seoul 135710, South KoreaChungnam Natl Univ, Dept Microbiol, Taejon 301747, South Korea
Kim, S. -Y.
Shin, A-R.
论文数: 0引用数: 0
h-index: 0
机构:
Chungnam Natl Univ, Dept Microbiol, Taejon 301747, South Korea
Chungnam Natl Univ, Res Inst Med Sci, Taejon 301747, South KoreaChungnam Natl Univ, Dept Microbiol, Taejon 301747, South Korea
Shin, A-R.
Kim, H. -J.
论文数: 0引用数: 0
h-index: 0
机构:
Chungnam Natl Univ, Dept Microbiol, Taejon 301747, South Korea
Chungnam Natl Univ, Res Inst Med Sci, Taejon 301747, South KoreaChungnam Natl Univ, Dept Microbiol, Taejon 301747, South Korea
Kim, H. -J.
Cho, S. -N.
论文数: 0引用数: 0
h-index: 0
机构:
Yonsei Univ, Coll Med, Dept Microbiol, Seoul 120752, South Korea
Yonsei Univ, Coll Med, Brain Korea Project Med Sci 21, Seoul 120752, South KoreaChungnam Natl Univ, Dept Microbiol, Taejon 301747, South Korea
Cho, S. -N.
Park, J. -K.
论文数: 0引用数: 0
h-index: 0
机构:
Chungnam Natl Univ, Dept Microbiol, Taejon 301747, South Korea
Chungnam Natl Univ, Res Inst Med Sci, Taejon 301747, South Korea
Chungnam Natl Univ, Coll Med, Canc Res Inst, Taejon 301747, South KoreaChungnam Natl Univ, Dept Microbiol, Taejon 301747, South Korea
机构:
Sun Yat Sen Univ, Inst Immunol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R ChinaSun Yat Sen Univ, Inst Immunol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China
Li, Jiangping
Shen, Juan
论文数: 0引用数: 0
h-index: 0
机构:
Sun Yat Sen Univ, Inst Immunol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R ChinaSun Yat Sen Univ, Inst Immunol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China
Shen, Juan
Lao, Suihua
论文数: 0引用数: 0
h-index: 0
机构:
Chest Hosp, Sect ICU, Guangzhou 510095, Guangdong, Peoples R ChinaSun Yat Sen Univ, Inst Immunol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China
Lao, Suihua
Li, Xiaomin
论文数: 0引用数: 0
h-index: 0
机构:
Sun Yat Sen Univ, Inst Immunol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R ChinaSun Yat Sen Univ, Inst Immunol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China
Li, Xiaomin
Liu, Jie
论文数: 0引用数: 0
h-index: 0
机构:
Sichuan Univ, West China Sch Med, West China Hosp, Lab Infect Dis & Vaccine, Chengdu 610041, Sichuan, Peoples R ChinaSun Yat Sen Univ, Inst Immunol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China
Liu, Jie
Wu, Changyou
论文数: 0引用数: 0
h-index: 0
机构:
Sun Yat Sen Univ, Inst Immunol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R ChinaSun Yat Sen Univ, Inst Immunol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China
机构:
Univ Delhi, Dept Life Sci, Acharya Narendra Dev Coll, New Delhi 110019, IndiaUniv Delhi, Dept Life Sci, Acharya Narendra Dev Coll, New Delhi 110019, India
Das, Ritam
论文数: 引用数:
h-index:
机构:
Eniyan, Kandasamy
Bajpai, Urmi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Delhi, Dept Biomed Sci, Acharya Narendra Dev Coll, New Delhi 110019, IndiaUniv Delhi, Dept Life Sci, Acharya Narendra Dev Coll, New Delhi 110019, India
机构:
Univ Estadual Feira de Santana, LMM, BR-44036900 Feira De Santana, BA, BrazilUniv Estadual Feira de Santana, LMM, BR-44036900 Feira De Santana, BA, Brazil
Cerqueira, E. J.
Santana, I. B.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Estadual Feira de Santana, LMM, BR-44036900 Feira De Santana, BA, BrazilUniv Estadual Feira de Santana, LMM, BR-44036900 Feira De Santana, BA, Brazil
Santana, I. B.
Silva, K. S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Estadual Feira de Santana, LMM, BR-44036900 Feira De Santana, BA, BrazilUniv Estadual Feira de Santana, LMM, BR-44036900 Feira De Santana, BA, Brazil
Silva, K. S.
Santos Junior, M. C., Jr.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Estadual Feira de Santana, LMM, BR-44036900 Feira De Santana, BA, BrazilUniv Estadual Feira de Santana, LMM, BR-44036900 Feira De Santana, BA, Brazil