Investigation of the Effects of Polyunsaturated Fatty Acid Ratios in Human SH-SY5Y Cells by in vitro Methods

被引：0
作者：
Elci, Mualla Pinar [1 ]
Fatsa, Tugba [2 ]
Oren, Sema [2 ]
Miser Salihoglu, Ece [3 ]
Akaydin, Sevgi [3 ]
机构：
[1] Univ Hlth Sci Turkiye, Gulhane Hlth Sci Inst, Stem Cell Lab, Ankara, Turkiye
[2] Univ Hlth Sci Turkiye, Gulhane Hlth Sci Inst, Mol Lab, Ankara, Turkiye
[3] Gazi Univ, Fac Pharm, Dept Biochem, Ankara, Turkiye
来源：
TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES | 2025年 / 22卷 / 01期
关键词：
Apoptosis; IL-6; NF-xB; ROS; SHSY-5Y cell; TGF beta 1; omega-3:omega-6 ratio; EICOSAPENTAENOIC ACID; DOCOSAHEXAENOIC ACID; INDUCE APOPTOSIS; NEUROBLASTOMA; CANCER; GROWTH; OMEGA-3-FATTY-ACIDS; N-3; SUPPLEMENTATION; PROLIFERATION;
D O I：
10.4274/tjps.galenos.2024.40583
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Objectives Neuroblastoma, an embryonic tumor of the sympathetic nervous system, is the deadliest type of cancer, accounting for 6-9% of all childhood cancers. The omega (omega)-6 [linoleic acid (LA)]: omega-3 [eicosapentaenoic acid (EPA)]=13/1 ratio is associated with the presence of chronic disease, and it has been reported that reducing this ratio to 7/1 protects against cancer and cardiovascular diseases. This study aimed to investigate the anticancer effects of different ratios of omega-3 and omega-6 fatty acids in human neuroblastoma cells (SHSY-5Y). Materials and Methods SHSY-5Y cells were treated with different ratios of omega-3 and omega-6 fatty acids for 48 and 72 h. The viability of omega-3 and omega-6 fatty acid-treated cells was measured using the methylthiazolyldiphenyl-tetrazolium bromide. The percentage of cell apoptosis was detected using the Fluorescein Isothiocyanate-conjugated Annexin-V/PI assay, and reactive oxygen species (ROS) analysis was performed using flow cytometry. The expression levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and transforming growth factor beta 1 (TGF beta 1) genes were determined using real-time polymerase chain reaction. Results EPA and LA separately significantly inhibited the proliferation of SH-SY5Y cells within 48 h (p<0.001). It was found that apoptosis decreased significantly in all groups due to the application of polyunsaturated fatty acids at different ratios, and the most effective dose was omega-3:omega-6 ratio: 1/1. ROS levels were significantly decreased compared with the control group and the lowest ROS level was observed in the omega-3:omega-6 ratio: 1/4 group. Both TNF-alpha, IL-6, and TGF beta 1 mRNA expressions increased significantly after the addition of fatty acid mixtures compared with the control; they were observed to decrease with an increasing omega-6 ratio. Conclusion This study is the first to examine the effects of the omega-3:omega-6 ratio on neuroblastoma cancer cells. The application of omega fatty acids decreased apoptosis at all ratios. In contrast, when the omega-3: omega-6 ratio increased, the amount of ROS. Additionally, as the omega-3:omega-6 ratio increases, a decrease in the release of pro-inflammatory cytokines is noted.
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页码：10 / 18
页数：9
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