A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging

被引:1
作者
Trozzo, Sophia [1 ,2 ]
Neupane, Bijita [1 ]
Foster, Paula J. [1 ,2 ]
机构
[1] Western Univ, Schulich Sch Med & Dent, Dept Med Biophys, London, ON N6A 3K7, Canada
[2] Western Univ, Robarts Res Inst, Schulich Sch Med & Dent, London, ON N6A 3K7, Canada
基金
加拿大健康研究院;
关键词
cell tracking; magnetic particle imaging; bioluminescence imaging; multimodal imaging; sensitivity; cell detection; quantification; LUCIFERASE REPORTER; CELLS; TRACKING; IRON; DELIVERY; BRAIN; MOUSE; MRI;
D O I
10.3390/tomography10110135
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Background: Preclinical cell tracking is enhanced with a multimodal imaging approach. Bioluminescence imaging (BLI) is a highly sensitive optical modality that relies on engineering cells to constitutively express a luciferase gene. Magnetic particle imaging (MPI) is a newer imaging modality that directly detects superparamagnetic iron oxide (SPIO) particles used to label cells. Here, we compare BLI and MPI for imaging cells in vitro and in vivo. Methods: Mouse 4T1 breast carcinoma cells were transduced to express firefly luciferase, labeled with SPIO (ProMag), and imaged as cell samples after subcutaneous injection into mice. Results: For cell samples, the BLI and MPI signals were strongly correlated with cell number. Both modalities presented limitations for imaging cells in vivo. For BLI, weak signal penetration, signal attenuation, and scattering prevented the detection of cells for mice with hair and for cells far from the tissue surface. For MPI, background signals obscured the detection of low cell numbers due to the limited dynamic range, and cell numbers could not be accurately quantified from in vivo images. Conclusions: It is important to understand the shortcomings of these imaging modalities to develop strategies to improve cellular detection sensitivity.
引用
收藏
页码:1846 / 1866
页数:21
相关论文
共 55 条
[1]   Magnetic Resonance Imaging of Tumor-Associated Macrophages: Clinical Translation [J].
Aghighi, Maryam ;
Theruvath, Ashok J. ;
Pareek, Anuj ;
Pisani, Laura L. ;
Alford, Raphael ;
Muehe, Anne M. ;
Sethi, Tarsheen K. ;
Holdsworth, Samantha J. ;
Hazard, Florette K. ;
Gratzinger, Dita ;
Luna-Fineman, Sandra ;
Advani, Ranjana ;
Spunt, Sheri L. ;
Daldrup-Link, Heike E. .
CLINICAL CANCER RESEARCH, 2018, 24 (17) :4110-4118
[2]   Multimodality Imaging of Bone Marrow-Derived Dendritic Cell Migration and Antitumor Immunity [J].
Ahn, Su-Bi ;
Lee, Sang Bong ;
Singh, Thoudam Debraj ;
Cho, Sung Jin ;
Kim, Sang Kyoon ;
Lee, In-Kyu ;
Jeong, Shin Young ;
Ahn, Byeong-Cheol ;
Lee, Jaetae ;
Lee, Sang-Woo ;
Jeon, Yong Hyun .
TRANSLATIONAL ONCOLOGY, 2017, 10 (02) :262-270
[3]   Tracking immune cells in vivo using magnetic resonance imaging [J].
Ahrens, Eric T. ;
Bulte, Jeff W. M. .
NATURE REVIEWS IMMUNOLOGY, 2013, 13 (10) :755-763
[4]   Firefly luciferase offers superior performance to AkaLuc for tracking the fate of administered cell therapies [J].
Amadeo, Francesco ;
Plagge, Antonius ;
Chacko, Anitta ;
Wilm, Bettina ;
Hanson, Vivien ;
Liptrott, Neill ;
Murray, Patricia ;
Taylor, Arthur .
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2022, 49 (03) :796-808
[5]   Size-dependent ferrohydrodynamic relaxometry of magnetic particle imaging tracers in different environments [J].
Arami, Hamed ;
Ferguson, R. M. ;
Khandhar, Amit P. ;
Krishnan, Kannan M. .
MEDICAL PHYSICS, 2013, 40 (07)
[6]   Uptake kinetics and biodistribution of 14C-D-luciferin-a radiolabeled substrate for the firefly luciferase catalyzed bioluminescence reaction: impact on bioluminescence based reporter gene imaging [J].
Berger, Frank ;
Paulmurugan, Ramasamy ;
Bhaumik, Srabani ;
Gambhir, Sanjiv Sam .
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2008, 35 (12) :2275-2285
[7]   Simultaneous imaging of widely differing particle concentrations in MPI: problem statement and algorithmic proposal for improvement [J].
Boberg, Marija ;
Gdaniec, Nadine ;
Szwargulski, Patryk ;
Werner, Franziska ;
Moeddel, Martin ;
Knopp, Tobias .
PHYSICS IN MEDICINE AND BIOLOGY, 2021, 66 (09)
[8]   19F-MRI for monitoring human NK cells in vivo [J].
Bouchlaka, Myriam N. ;
Ludwig, Kai D. ;
Gordon, Jeremy W. ;
Kutz, Matthew P. ;
Bednarz, Bryan P. ;
Fain, Sean B. ;
Capitini, Christian M. .
ONCOIMMUNOLOGY, 2016, 5 (05)
[9]   Akaluc bioluminescence offers superior sensitivity to track in vivo glioma expansion [J].
Bozec, Dominique ;
Sattiraju, Anirudh ;
Bouras, Alexandros ;
Raj, Joe G. Jesu ;
Rivera, Daniel ;
Huang, Yong ;
Alves, Chrystian Junqueira ;
Tejero, Rut ;
Tsankova, Nadejda M. ;
Zou, Hongyan ;
Hadjipanayis, Constantinos ;
Friedel, Roland H. .
NEURO-ONCOLOGY ADVANCES, 2020, 2 (01)
[10]   Clinical Tracking of Cell Transfer and Cell Transplantation: Trials and Tribulations [J].
Bulte, Jeff W. M. ;
Daldrup-Link, Heike E. .
RADIOLOGY, 2018, 289 (03) :604-615