Bioinspired PCL-based composite scaffolds produced via hot melt extrusion and fused filament fabrication: An integrated workflow for enhanced bone regeneration

被引：0
作者：
Tommasino, Carmela [1 ,2 ]
Sardo, Carla [1 ]
Guidone, Angiola [1 ,2 ]
Soriente, Alessandra [3 ]
Raucci, Maria Grazia [3 ]
Ciaglia, Tania [1 ]
Auriemma, Giulia [1 ]
Aquino, Rita Patrizia [1 ]
机构：
[1] Univ Salerno, Dept Pharm, Via Giovanni Paolo 2 132, I-84084 Fisciano, SA, Italy
[2] Univ Salerno, PhD Program Drug Discovery & Dev, Via Giovanni Paolo 2 132, I-84084 Fisciano, SA, Italy
[3] Natl Res Council Italy IPCB CNR, Inst Polymers Composites & Biomat, Viale John Fitzgerald Kennedy 54,Mostra Oltremare, I-80125 Naples, Italy
来源：
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY | 2025年 / 106卷
关键词：
3D printing; Fused filament fabrication; Hot melt extrusion; Bioinspired PCL composites; Nature-derived fillers; Bone tissue engineering; Drug-eluting scaffolds; POLYCAPROLACTONE SCAFFOLDS; IN-VITRO; MODEL;
D O I：
10.1016/j.jddst.2025.106679
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
This study presents a comprehensive and integrated workflow for fabricating bioinspired, macroporous 3D scaffolds tailored for bone tissue engineering. Poly-(epsilon-caprolactone) (PCL) served as the base polymer, enriched with nature-derived fillers such as sodium alginate and microcrystalline cellulose, along with bioactive nanohydroxyapatite ceramics, to enhance hydrophilicity, bioactivity, and cellular interactions. A combined manufacturing approach was implemented to ensure uniform filler distribution and optimized scaffold performance: hybrid pellets preparation and composite filament production through Hot Melt Extrusion (HME), followed by scaffold fabrication using Fused Filament Fabrication (FFF). This multistep process allowed precise control over material composition, ensuring compatibility and consistency across fabrication stages. The 3D printed scaffolds were characterized for printability, architecture, surface topography, thermal and mechanical properties, degradation behavior, swelling capacity, and drug release profiles. Incorporating nature- derived fillers improved hydrophilicity, swelling behavior, and biodegradation kinetics, while nanohydroxyapatite enhanced mechanical strength and sustained release of dexamethasone. In vitro tests using murine pre-osteoblasts confirmed scaffold biocompatibility and osteogenic potential, promoting cell proliferation and differentiation. The findings underscore the potential of this integrated workflow for advancing scaffold design and bone regeneration.
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页数：22
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