A new β-amylase detection strategy based on encapsulated enzyme magnetic layered double hydroxide with high sensitivity and simplified workflow

(3-Amylase (BMY) is a linchpin in food production and the pharmaceutical industry because the enzyme efficiently controls the ratio of diverse saccharides in fermentation and the manufacture of high-quality maltose. However, existing BMY detection tactics suffer from inadequate selectivity/sensitivity and cumbersome operation and do not meet the needs of precise quantification. Consequently, there is an urgent need to develop an ultrasensitive sensing platform to achieve precise BMY analysis with a low detection limit and simpler workflow. In this work, we establish an encapsulated-enzyme-based BMY biosensing platform in which alpha-glucosidase is embedded in magnetic layered double hydroxide using a self-sacrificing template. The encapsulated enzyme has increased activity, robustness, and recyclability and was utilized for BMY detection via a cascade chromatic process. We found a detection limit for the quantification of BMY activity of 2.67 U/L with a broad range (5-400 U/L), fast response speed (10 min), and satisfactory specificity. We applied the biosensing platform to liquor starters to verify the capability of the assay in complicated fermentation samples. The proposed platform holds great promise as an efficient and simple method for enzymatic bioactivity monitoring in food manufacturing, biopharmaceutical processing, and clinical laboratory tests.