Cloning and sequence analysis of goat GSK3B gene and its effect on lipogenesis in goat intramuscular adipocytes

The aim of this study was to detect the expression of GSK3B gene in different tissues of goats and the expression level of intramuscular precursor adipocytes at different differentiation periods, and to further reveal the effects of overexpression and interferences of GSK3B gene on lipid deposition in intramuscular precursor adipocytes of goats. The results showed that a nucleotide sequence of 1,433 bp, CDS region 1,263 bp, encoding 420 amino acids, was obtained for the goat GSK3B gene, Goat GSK3B gene expression was highest in the lungs, lowest in the longest dorsal muscle, and highest at 120 h of induced differentiation. Overexpression of GSK3B gene significantly reduced lipid droplets in intramuscular precursor adipocytes, and also significantly down-regulated the relative expression of SREBP1, CEBP beta and PPAR gamma (p < 0.05), with no significant changes in the expression of FASN, CEBP alpha, SCD and LPL (p > 0.05), GSK3B inhibition increased lipid droplets accumulation by significantly down-regulated SREBP1, CEBP beta and PPAR gamma expression (p < 0.05). In conclusion, GSK3B expression significantly inhibited lipid deposition in goat intramuscular precursor adipocytes. These results provide important data to further elucidate the molecular mechanisms regulating intramuscular fat formation in goats.