Pro-angiogenic effects of Guo Min decoction in a zebrafish model

被引:0
作者
Lu, Ping-Hsun [1 ]
Tung, Sheng-Fen [2 ]
Wen, Chi-Chung [3 ]
Huang, Ching-Yuan [2 ]
Huang, Jhih-Wei [2 ]
Chen, Chih-Hsin [2 ]
Chen, Yau-Hung [2 ]
机构
[1] Buddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Chinese Med, New Taipei, Taiwan
[2] Tamkang Univ, Dept Chem, 151 Yingzhuan Rd, New Taipei, Taiwan
[3] Tamkang Univ, Dept Math, New Taipei, Taiwan
来源
TZU CHI MEDICAL JOURNAL | 2025年 / 37卷 / 01期
关键词
Angiogenesis; Guo Min decoction; Real-time polymerase chain reaction; Zebrafish; CELLS;
D O I
10.4103/tcmj.tcmj_59_24
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objectives:Guo Min decoction (GMD) is a Chinese traditional medicine that can regulate allergy-related symptoms. Although GMD treatment was reported to treat allergy-associated symptoms by regulating the immune response, the rationale between GMD treatment and angiogenesis has not been reported yet. Our objective is to investigate the angiogenesis-modulating activity of GMD.Materials and Methods:In this study, we used fluorescence recording, alkaline phosphatase (AP) activity staining, and real-time polymerase chain reaction (PCR) experiments to examine the effects of GMD on angiogenesis in a zebrafish model.Results:GMD-treated zebrafish embryos exhibited more intercapillary spaces in the caudal vein plexus (Mock: 11.1 +/- 1.8 [n = 20; n: numbers of embryos]; GMD-treated: 16.2 +/- 1.9 [n = 20]). AP activity staining showed that treatment with GMD and liquorice (Gan Cao, a component of GMD) induced subintestinal vein outgrowth. However, glycyrrhizin (a component of Gan Cao) had no obvious pro-angiogenic effects on zebrafish. Furthermore, real-time PCR experiments indicated that GMD exposure might be through regulating angiogenesis-related genes (cdh5, nrp1a, and flt1) expressions.Conclusion:Based on these observations, we proposed that GMD had pro-angiogenic activity in a zebrafish model, and it might partially be contributed by one of the components, liquorice.
引用
收藏
页码:58 / 64
页数:7
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