Neofusicoccum mangiferae causing leaf spot disease of Eucalyptus: : First report from India

Fungal diseases are a serious threat to Eucalyptus spp., which are cultivated worldwide for pulp and paper. Robust and timely detection of Eucalyptus pathogens is thus a crucial step in disease prevention and control. In 2022<bold>,</bold> leaf spots were observed on Eucalyptus trees in Karnal, northern India. Fungal isolates displaying morphological characteristics similar to Neofusicoccum mangiferae were consistently recovered from the infected leaves. The morphological identification was validated by phylogenetic analysis employing internal transcribed spacer (ITS), partial translation elongation factor-1 alpha (tef1) and partial beta-tubulin (tub2) regions. Finally, Koch's postulates for N. mangiferae as a foliar pathogen of Eucalyptus were fulfilled. This is the first confirmed report of N. mangiferae affecting Eucalyptus trees in India.