Properties And Antimicrobial Activity of Bacterial Nanocellulose Composite Incorporated with Silver Nanoparticles Based on Green Synthesis

Green silver nanoparticles (Ag NPs) synthesized using medical plant extracts as capping and reducing agents demonstrate broad applicability in addressing the threat posed by infections. In this study, we present a sustainable, low-toxicity, and cost-effective bacterial nanocellulose composite with silver nanoparticles based on green synthesis that uses Origanum vulgare as a reducing agent due to numerous biological components. The formation of Ag NPs in composite and morphological properties was analyzed using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and transmission electron microscopy (TEM) techniques. Antimicrobial activity was determined using the agar diffusion Kirby-Bauer method against Gram-positive and Gram-negative bacterial strains. The antimicrobial activity of bacterial nanocellulose composite/Ag NPs was substantially strong against all tested bacterial cultures. The structural investigation of Ag NPs by TEM presents the presence of spherical-shaped silver nanoparticles in cellulose composite. This method of obtaining antimicrobial composites could be widely used to fight against antibiotics, antibiotic-resistant antibiotic-resistant bacteria, and other Gram-positive and Gram-negative bacteria.