Small extracellular vesicles derived from auricular chondrocytes promote secretion of interleukin 10 in bone marrow M1-like

被引：1

作者：

Kobatake, Tetsuya ^{[1
]}

Miyamoto, Yoshiyuki ^{[2
,3
]}

Fujihara, Yuko ^{[4
]}

Saijo, Hideto ^{[3
,5
]}

Hoshi, Kazuto ^{[3
,6
]}

Hikita, Atsuhiko ^{[6
,7
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Sensory & Motor Syst Med, Hongo 7-3-1,Bunkyo Ku, Tokyo 1138655, Japan

[2] Mitsui Mem Hosp, Div Dent & Oral Surg, Kanda Izumi Cho 7,Chiyoda Ku, Tokyo 7078643, Japan

[3] Univ Tokyo Hosp, Dept Oral Maxillofacial Surg & Orthodont, Hongo 7-3-7,Bunkyo Ku, Tokyo 7738655, Japan

[4] Tokyo Teishin Hosp, Dept Dent & Oral Surg, 2-74-23 Fujimi,Chiyoda Ku, Tokyo 7028798, Japan

[5] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Oral & Maxillofacial Surg, Field Oral & Maxillofacial Rehabil,Adv Therapeut C, 8-35-7 Sakuragaoka, Kagoshima 8908544, Japan

[6] Univ Tokyo Hosp, Div Tissue Engn, Hongo 7-3-7,Bunkyo Ku, Tokyo 7738655, Japan

[7] Univ Tokyo Hosp, Clin Stem Cell Biol, Hongo 7-3-7,Bunkyo Ku, Tokyo 7738655, Japan

来源：

REGENERATIVE THERAPY | 2025年 / 28卷

基金：

日本学术振兴会;

关键词：

Macrophages; Chondrocytes; Extracellular vesicles; Paracrine; Anti-inflammatory cytokine; CARTILAGE; IL-10; OSTEOARTHRITIS; POLARIZATION; CSF;

D O I：

10.1016/j.reth.2025.01.009

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Introduction: Elucidation of the paracrine interaction between chondrocytes and macrophages is useful for understanding the mechanisms of cartilage regeneration. Extracellular vesicles are granular substances with a diameter of approximately 150 nm, surrounded by a phospholipid bilayer membrane. In recent years, research has been conducted on clinical applications of extracellular vesicles. It has been shown that macrophages promote cartilage maturation, and macrophages acquire anti-inflammatory properties through cartilage, but the detailed mechanism of paracrine action involving extracellular vesicles remains unclear. Therefore, we focused on the effect of chondrocyte-derived extracellular vesicles on changes in macrophage characteristics. Methods: Macrophages induced with granulocyte-macrophage colony stimulating factor (M1-like macrophages) and auricular chondrocytes were co-cultured using cell culture inserts and exosome inhibitors, and the expression of macrophage markers were analyzed. Next, extracellular vesicles separated from auricular chondrocytes were added to in vitro macrophage culture medium, and time-lapse observations of macrophage uptake of auricular chondrocyte-derived extracellular vesicles were performed. In addition, the effects of extracellular vesicles on the expression of macrophage markers were also analyzed. Results: The expression of CD206, an M2 macrophage marker, was increased in macrophages due to the paracrine effect of chondrocytes, and CD206 expression was further increased by pharmacological inhibition of chondrocyte-derived exosomes. It was shown that chondrocyte-derived extracellular vesicles were taken up by macrophages and promoted the production of interleukin-10, an anti-inflammatory cytokine while reducing CD206 expression. Conclusions: Auricular chondrocyte-derived extracellular vesicles promoted the production of interleukin-10 in bone marrow M1-like macrophages but reduced CD206 expression. (c) 2025 The Author(s). Published by Elsevier BV on behalf of The Japanese Society for Regenerative Medicine. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/lice nses/by-nc-nd/4.0/).

引用

页码：421 / 430

页数：10

共 45 条

[1] Chondrogenic potential of IL-10 in mechanically injured cartilage and cellularized collagen ACI grafts [J].