Genetic Transformation of Triticum dicoccum and Triticum aestivum with Genes of Jasmonate Biosynthesis Pathway Affects Growth and Productivity Characteristics

被引:0
作者
Miroshnichenko, Dmitry N. [1 ,2 ]
Pigolev, Alexey V. [1 ]
Pushin, Alexander S. [2 ]
Alekseeva, Valeria V. [2 ]
Degtyaryova, Vlada I. [2 ]
Degtyaryov, Evgeny A. [1 ]
Pronina, Irina V. [3 ]
Frolov, Andrej [4 ]
Dolgov, Sergey V. [2 ]
Savchenko, Tatyana V. [1 ]
机构
[1] Russian Acad Sci, Pushchino Sci Ctr Biol Res, Inst Basic Biol Problems, Pushchino 142290, Russia
[2] Russian Acad Sci, Branch Shemyakin, Ovchinnikov Inst Bioorgan Chem, Pushchino 142290, Russia
[3] State Univ Educ, Dept Physiol Human Ecol & Med & Biol Sci, Mytishi 141014, Russia
[4] Russian Acad Sci, Timiryazev Inst Plant Physiol, Lab Analyt Biochem & Biotechnol, Moscow 127276, Russia
来源
PLANTS-BASEL | 2024年 / 13卷 / 19期
基金
俄罗斯科学基金会;
关键词
particle inflow gun; bread wheat; emmer wheat; jasmonates; allene oxide synthase; 12-oxophytodienoate reductase; SOMATIC EMBRYOGENESIS; SIGNAL-TRANSDUCTION; ACID; WHEAT; ARABIDOPSIS;
D O I
10.3390/plants13192781
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The transformation protocol based on the dual selection approach (fluorescent protein and herbicide resistance) has been applied here to produce transgenic plants of two cereal species, emmer wheat and bread wheat, with the goal of activating the synthesis of the stress hormone jasmonates by overexpressing ALLENE OXIDE SYNTHASE from Arabidopsis thaliana (AtAOS) and bread wheat (TaAOS) and OXOPHYTODIENOATE REDUCTASE 3 from A. thaliana (AtOPR3) under the strong constitutive promoter (ZmUbi1), either individually or both genes simultaneously. The delivery of the expression cassette encoding AOS was found to affect morphogenesis in both wheat species negatively. The effect of transgene expression on the accumulation of individual jasmonates in hexaploid and tetraploid wheat was observed. Among the introduced genes, overexpression of TaAOS was the most successful in increasing stress-inducible phytohormone levels in transgenic plants, resulting in higher accumulations of JA and JA-Ile in emmer wheat and 12-OPDA in bread wheat. In general, overexpression of AOS, alone or together with AtOPR3, negatively affected leaf lamina length and grain numbers per spike in both wheat species. Double (AtAOS + AtOPR3) transgenic wheat plants were characterized by significantly reduced plant height and seed numbers, especially in emmer wheat, where several primary plants failed to produce seeds.
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页数:19
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