De Novo Transcriptome Profiling of Mustard Aphid (Lipaphis erysimi) and Differential Expression of Transcripts Associated with Feeding and Non-Feeding Conditions and Developmental Stages

被引：0

作者：

Chongtham, Rubina ^{[1
,2
]}

Sharma, Manvi ^{[1
]}

Shukla, Rohit Nandan ^{[3
]}

Joshi, Gopal ^{[1
]}

Kumar, Amar ^{[1
]}

Goel, Shailendra ^{[1
]}

Agarwal, Manu ^{[1
]}

Jagannath, Arun ^{[1
]}

机构：

[1] Univ Delhi, Dept Bot, Delhi 110007, India

[2] Univ Delhi, Deshbandhu Coll, Dept Bot, Delhi 110019, India

[3] Bionivid Technol Pvt Ltd, Kasturi Nagar, Bengaluru, India

来源：

INSECTS | 2024年 / 15卷 / 09期

关键词：

Lipaphis erysimi; transcriptome; feeding; non-feeding; adult; nymph; effectors; BLACK BEAN APHID; BREVICORYNE-BRASSICAE; GENE-EXPRESSION; MYZUS-PERSICAE; CABBAGE APHID; PLANT DEFENSE; PEA APHID; PROTEIN; HOST; EVOLUTION;

D O I：

10.3390/insects15090682

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Lipaphis erysimi is a specialist aphid of the Indian subcontinent that causes significant yield losses in oilseed Brassicas. Several aphid genes have been used as preferred targets in RNAi-based transgenic plants for aphid resistance. In order to enhance the repertoire of potential target genes for aphid control and to identify the genes associated with aphid feeding and development, we performed a two-way comparative study of differential gene expression profiles between (i) feeding and non-feeding adults and (ii) adult and nymph developmental stages of L. erysimi. De novo RNA-seq of aphids using Illumina technology generated a final transcriptome comprising 52,652 transcripts. Potential transcripts for host selection, detoxification, salivary proteins and effectors, molecular chaperones and developmental genes were identified. Differential gene expression studies identified variations in the expression of 1502 transcripts between feeding and non-feeding adults and 906 transcripts between nymphs and adults. These data were used to identify novel target genes for RNAi-based aphid control and facilitate further studies on the molecular basis of aphid feeding and development.

引用

页数：18

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