Protocol 4D light sheet imaging, computational reconstruction, and cell tracking in mouse embryos

被引：0

作者：

Dominguez, Martin H. ^{[1
,2
,3
]}

Muncie-Vasic, Jonathon M. ^{[1
]}

Bruneau, Benoit G. ^{[1
,4
,5
,6
]}

机构：

[1] Gladstone Inst, San Francisco, CA 94158 USA

[2] Univ Calif San Francisco, Dept Med, Div Cardiol, San Francisco, CA 94115 USA

[3] Univ Penn, Cardiovasc Inst, Philadelphia, PA 19104 USA

[4] Roddenberry Ctr Stem Cell Biol & Med Gladstone, San Francisco, CA 94158 USA

[5] Univ Calif San Francisco, Cardiovasc Res Inst, Inst Human Genet, Dept Pediat, San Francisco, CA 94158 USA

[6] Univ Calif San Francisco, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell R, San Francisco, CA 94158 USA

来源：

STAR PROTOCOLS | 2025年 / 6卷 / 01期

关键词：

MORPHOGENESIS;

D O I：

10.1016/j.xpro.2024.103515

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

As light sheet fluorescence microscopy (LSFM) becomes widely available, reconstruction of time-lapse imaging will further our understanding of complex biological processes at cellular resolution. Here, we present a comprehensive workflow for in toto capture, processing, and analysis of multi-view LSFM experiments using the ex vivo mouse embryo as a model system of development. Our protocol describes imaging on a commercial LSFM instrument followed by computational analysis in discrete segments, using open-source software. Quantification of migration and morphodynamics is included. For complete details on the use and execution of this protocol, please refer to Dominguez et al.1

引用

页数：57

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