High-order super-resolution optical fluctuation imaging with ultrasmall polymer dots

被引:0
作者
Gong, Mingyue [1 ]
Wu, Zhuli [1 ]
Liu, Jie [1 ]
Fang, Xiaofeng [1 ]
Yao, Zihan [1 ]
Wu, Changfeng [1 ]
机构
[1] Southern Univ Sci & Technol, Dept Biomed Engn, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Peoples R China
基金
中国国家自然科学基金;
关键词
SOFI;
D O I
10.1364/OL.545034
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Super-resolution optical fluctuation imaging (SOFI) rapidly generates super-resolution images by analyzing fluorescence intensity fluctuations. However, fluorophores for high-order SOFI applications are very rare. Here, we report ultrasmall semiconducting polymer dots (Pdots) to achieve high-order SOFI at single-particle and cellular levels. The ultrasmall Pdots exhibit an average diameter of -7 nm and superior photoblinking characteristics. The Pdot bioconjugates specifically labeled subcellular structures and demonstrated super-resolution imaging with a spatial resolution of -64 nm, which is -6 times enhancement compared to wide-field images. This study demonstrates the potential of the small-sized semiconductor Pdots for high-order SOFI gence (AI) training, and similar technologies, are reserved.
引用
收藏
页码:439 / 442
页数:4
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