Tomato B-cell lymphoma2 (Bcl2)-associated athanogene 5 (SlBAG5) contributes negatively to immunity against necrotrophic fungus Botrytis cinerea through interacting with SlBAP1 and modulating catalase activity

被引：0
作者：
Wang, Jiali [1 ,2 ]
Dai, Yujie [1 ,2 ]
Li, Xiaodan [1 ,2 ]
Zhu, Liya [1 ,2 ]
Liu, Shixia [1 ,2 ,3 ]
He, Yeling [1 ,2 ]
Zhang, Jing [4 ]
Song, Fengming [1 ,2 ]
Li, Dayong [1 ,2 ]
机构：
[1] Zhejiang Univ, Inst Biotechnol, Minist Agr & Rural Affairs, Key Lab Mol Biol Crop Pathogens & Insect Pests, Hangzhou 310058, Peoples R China
[2] Zhejiang Univ, Inst Biotechnol, Zhejiang Key Lab Biol & Ecol Regulat Crop Pathogen, Hangzhou 310058, Peoples R China
[3] Ningbo Univ, Sch Marine Sci, Key Lab Marine Biotechnol Zhejiang Prov, Ningbo 315211, Peoples R China
[4] Zhejiang Prov Ctr Dis Control & Prevent, Hangzhou 310057, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2025年 / 301卷
基金：
中国国家自然科学基金;
关键词：
Tomato; B cell lymphoma 2 (Bcl-2)-associated; athanogene protein; BON1-associated protein1; Catalase; Immune response; Botrytis cinerea; PAMP-TRIGGERED IMMUNITY; NADPH OXIDASE RBOHD; BCL-2-ASSOCIATED ATHANOGENE; TRANSIENT EXPRESSION; REGULATES DEFENSE; PLANT; ARABIDOPSIS; KINASE; BAP1; FAMILY;
D O I：
10.1016/j.ijbiomac.2025.140466
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The evolutionarily conserved and multifunctional B-cell lymphoma2 (Bcl2)-associated athanogene proteins (BAGs), serving as co-chaperone regulators, play a pivotal role in orchestrating plant stress responses. In this study, the possible involvement of tomato SlBAG genes in resistance to Botrytis cinerea was examined. The SlBAG genes respond with different expression change patterns to B. cinerea and defense signaling hormones. SlBAG proteins are individually differentially localized to the nucleus, mitochondria, cytoplasm, endoplasmic reticulum (ER), or vacuole. Silencing of SlBAG5 enhanced immunity to B. cinerea, while overexpression weakened it, affecting Botrytis-induced JA/ET defense gene expression and JA levels. Chitin-induced ROS burst and expression of PTI marker genes SlPTI5 and SlLRR22 were strengthened in SlBAG5-silenced plants but were weakened in SlBAG5-overexpressing plants (SlBAG5-OE) plants. SlBAG5 interacts with BON1 ASSOCIATED PROTEIN 1 (SlBAP1) through its BAG domain, and the stability of SlBAP1 depends on the presence of SlBAG5. Silencing of SlBAP1 conferred increased resistance to B. cinerea through increased expression of JA/ET signaling and defense genes. SlBAP1 functions by recruiting and boosting SlCAT3 activity to remove H2O2. The findings suggest that SlBAG5 suppresses tomato immunity to B. cinerea by stabilizing SlBAP1, which modulates ROS scavenging and acts as a negative regulator of immunity.
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页数：16
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