THE CORRELATION BETWEEN BRAFV600E EXPRESSION AND INTEGRIN β 3 IN MELANOMA CELLS

This study investigated the relationship between ITGB3 and BRAFV600E expression in melanoma. It specifically focused on how the overexpression of BRAFV600E and Integrin /33 impacts wound healing and the TGF-/3 pathway. The findings provide valuable insights into melanoma progression and highlight potential therapeutic targets. Homozygous BRAFV600E-carrying A375 and heterozygous BRAFV600Ecarrying M14 melanoma cell lines were utilized. ITGB3 mRNA levels were measured in both A375 and M14 cells, while Integrin /33 protein levels were analyzed in BRAFV600E-overexpressing A375 cells. The impact of increased BRAFV600E and Integrin /33 on cell migration was assessed using an in vitro wound healing assay. Additionally, the influence of BRAFV600E and Integrin /33 overexpression on the TGF-/3 pathway was evaluated through luciferase reporter assays and real-time PCR. ITGB3 levels were significantly higher in A375 cells compared to M14 cells. Overexpression of BRAFV600E in A375 cells resulted in a marked increase in Integrin /33 levels. Cells overexpressing both BRAFV600E and Integrin /33 demonstrated enhanced wound healing rates and elevated TGF-/3 activity. These findings suggest a strong correlation between BRAFV600E expression and Integrin /33 levels in melanoma cells. This study uncovers a significant relationship between BRAFV600E expression and Integrin /33 levels in melanoma cells. The overexpression of both BRAFV600E and Integrin /33 enhances wound healing and promotes TGF-/3 signalling. These findings suggest that Integrin /33 may contribute to melanoma progression and poor prognosis by influencing cell migration and the TGF-/3 pathway. Targeting integrins, the TGF-/3 pathway, and BRAFV600E present potential therapeutic strategies for melanoma treatment.