Efficacy of cold and cryo-preserved nerve allografts with low-dose FK506 for motor nerve regeneration: a preclinical study

被引:0
作者
Kim, Jong Pil [1 ]
Heo, Soon Chul [2 ,3 ]
Lee, Dae Hee [4 ]
Bae, Jun Sang [5 ,6 ]
Shin, Young Kwang [4 ]
Son, Su Hyeok [4 ]
Park, Il Yong [11 ]
Kim, Hae-Won [2 ,3 ,7 ,8 ,9 ,10 ]
Lee, Jun Hee [2 ,3 ,7 ,8 ,9 ]
Kim, Kyung Wook [4 ]
机构
[1] Naeunpil Hosp, Dept Orthopaed Surg, Cheonan, South Korea
[2] Dankook Univ, Inst Tissue Regenerat Engn ITREN, 119 Dandae Ro, Cheonan 31116, South Korea
[3] Dankook Univ, Mechanobiol Dent Med Res Ctr, Cheonan 31116, South Korea
[4] Dankook Univ, Dankook Univ Hosp, Coll Med, Dept Orthopaed Surg, 201,Manghyang Ro, Cheonan Si, South Korea
[5] Dankook Univ, Coll Med, Beckman Laser Inst Korea, Cheonan, South Korea
[6] Dankook Univ, Med Laser Res Ctr, Cheonan, South Korea
[7] Dankook Univ, Dept Nanobiomed Sci, Cheonan 31116, South Korea
[8] Dankook Univ, Four NBM Global Res Ctr Regenerat Med BK21, Cheonan 31116, South Korea
[9] Dankook Univ, Coll Dent, Dept Biomat Sci, Cheonan 31116, South Korea
[10] Dankook Univ, UCL Eastman Korea Dent Med Innovat Ctr, Cheonan 31116, South Korea
[11] Dankook Univ, Coll Med, Dept Biomed Engn, Cheonan 31116, South Korea
来源
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH | 2024年 / 19卷 / 01期
基金
新加坡国家研究基金会;
关键词
Cryopreservation; Cold preservation; Allograft; Immunosuppression; Nerve reconstruction; FK506; AXONAL REGENERATION; FUNCTIONAL RECOVERY; SCHWANN-CELLS; RAT; REPAIR; DEPENDENCE; CONDUITS; GRAFTS; GAP; NEUROREGENERATION;
D O I
10.1186/s13018-024-05343-1
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
BackgroundDespite their ability to regenerate as well as autografts, the use of nerve allografts is limited by the need for immunosuppression and the risk of disease transmission. Further, decellularized allografts lacking Schwann cells limit axonal regeneration in long nerve defects. This study evaluated sciatic nerve regeneration in rats implanted with cold- or cryopreserved allografts, and examined the effects of FK506, an immunosuppressant that targets calcineurin function, on motor recovery.MethodsSixty-five male Lewis rats were divided into five groups of 13, each with a 10-mm sciatic nerve gap. Group I received an autograft, whereas Groups II and III received allografts pretreated with cryopreservation and cold preservation, respectively. Groups IV and V were also implanted with cryo- and cold-preserved allografts, but were treated with a low dose of FK506. Motor regeneration was assessed at 20 weeks by the measurement of ankle contracture, compound muscle action potential, maximal isometric tetanic force, wet muscle weight of the tibialis anterior, peroneal nerve histomorphometry, and immunohistochemistry of the reconstructed sciatic nerve.ResultsSimilar motor recovery was observed between the autografts and both types of allografts. The groups treated with FK506 showed improved recovery, particularly in terms of ankle angle and tibialis anterior muscle weight. Histomorphometry revealed a superior myelinated fiber area and nerve ratio in the cold-preserved allograft group, while Group II displayed a less well-organized morphology.ConclusionThis study demonstrates that cold- or cryopreserved nerve allografts represent effective alternatives to autografts for peripheral nerve reconstruction, with low-dose FK506 enhancing motor recovery without necessitating immunosuppression.Level of Evidence IBasic Science Level I.
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页数:13
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