Protocol for a low-volume, direct analysis urine preparation procedure for non-targeted GC-MS metabolomics

被引：0
作者：
Allen, Bianca [1 ]
Luies, Laneke [1 ]
机构：
[1] North West Univ, Focus Area Human Metabol, Potchefstroom Campus, ZA-2520 Potchefstroom, South Africa
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 04期
基金：
新加坡国家研究基金会;
关键词：
Chemistry; Health Sciences; Mass Spectrometry; Metabolism; Metabolomics;
D O I：
10.1016/j.xpro.2024.103449
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We present a low-volume, direct analysis protocol for non-targeted gas chromatography-mass spectrometry (GC-MS) metabolomics, using 100 m L of urine. The steps include sample collection, stock solution preparation, metabolite extraction, two-step derivation with a drying phase, and analysis via two-dimensional GC time-of-flight MS (GCxGC-TOFMS). This protocol improves the efficiency and thoroughness of urinary metabolite analysis, contributing to advancements in metabolomics research, disease diagnosis, and biomarker discovery. For complete details on the use and execution of this protocol, please refer to Olivier et al.1
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页数：21
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