Mechanism of Bacillus cooperating with silicon to re-balance chlorophyll metabolism and restore carbon metabolism of Glycyrrhiza uralensis Fisch. Seedlings exposed to salt-drought stress

Salt-drought is a major environmental event affecting crop productivity and quality by causing chlorophyll (Chl) and carbon balance disorder. There has been growing interest in the application of endophyte and silicon (Si), as inoculants for saline and drought land restoration. This study investigates the impact of Bacillus (Bs), Si, and Bs + Si on the disorder of Chl metabolism and carbon balance of G. uralensis seedlings under salt-drought stress (SD). Results showed that both Bs and Si treatments enhanced Chl and carbon metabolism, with the combined Bs and Si treatment showing a synergistic effect. Specifically, Bs + Si enhanced the mutual conversion of Chl a and Chl b, restored the equilibrium in Chl a and Chl b content, and increased RuBisco activity by 31.07%, thereby promoting carbon fixation. Subsequently, Bs + Si re-balanced the carbohydrate content, by increasing the sucrose synthase (SS), and beta-amylase (BMY) activities by 49.57%, and 83.59% respectively, and decreasing sucrose phosphate synthase (SPS), and granule-bound starch synthase (GBSS) activities by 38.93%, 40.93% respectively etc involved in the metabolism of sucrose and starch. Furthermore, Bs + Si facilitated the restoration of the typical progression of the tricarboxylic acid (TCA) cycle and glycolysis pathway (EMP). These findings highlight the synergistic role of Bs and Si in enhancing the salt and drought resilience of G. uralensis seedlings, offering promising strategies for sustainable agriculture, improving crop resilience to climate change, and achieving the "dual carbon" goals of carbon peaking and carbon neutrality.