Effects of Culture System and Substrate Composition on Micropropagated Plantlets of Two Varieties of Stevia rebaudiana Bert

被引:8
作者
Vilarino, Susana [1 ]
Florido, Maria del Carmen [2 ]
Garcia, Jose Luis [3 ]
Cantos, Manuel [3 ]
机构
[1] Vitrosur Lab SLU, Algodonera Sur Palacios, Seville 41720, Spain
[2] Univ Seville, Fac Chem, Dept Crystallog Mineral & Agr Chem, C-Prof Garcia Gonzalez 1, Seville 41012, Spain
[3] Inst Nat Resources & Agrobiol Seville CSIC, Seville 41012, Spain
来源
PHYSIOLOGIA | 2023年 / 3卷 / 01期
关键词
temporal immersion system; productivity; Criolla; Morita; MDA; TEMPORARY IMMERSION SYSTEMS; GROWTH; PROPAGATION; IMPROVEMENT; TOLERANCE;
D O I
10.3390/physiologia3010006
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Background: Secondary metabolites in stevia leaves offer important therapeutic benefits. The germination problems of stevia seeds and the high heterogeneity of the resulting populations make micropropagation the preferred tool for obtaining a large number of plants in a short time. Until now, studies have focused on optimizing the action of growth regulators for the improvement of stevia micropropagated plantlets. In this project, we study alternative mineral nutrients in the substrate and two culture systems on micropropagated stevia plantlets to obtain proper amounts of enhanced plantlets. Methods: Criolla and Morita varieties; MS, MSM, and G substrates, and temporary immersion (TIS) and agar-medium systems were used. Biometric variables and damage to the cell membrane of the resulting plantlets were tested. Results: More productivity in the Criolla plantlets growing in MS solid medium and TIS. The Morita plantlets presented higher productivity in the solid MS, MSM, and G substrates and TIS. The explants growing in MS and MSM presented the highest productivity. The solid substrate was generally better than TIS. No damage to the cell membrane was found. Conclusions: The micropropagation efficiency of stevia plantlets of the two varieties has been enhanced without negative effects on their physiological condition. Consequently, any of the culture systems used, including bioreactors, can be of industrial application on a large scale.
引用
收藏
页码:74 / 85
页数:12
相关论文
共 48 条
  • [11] DROUGHT TOLERANCE IN 2 MOSSES - CORRELATED WITH ENZYMATIC DEFENSE AGAINST LIPID-PEROXIDATION
    DHINDSA, RS
    MATOWE, W
    [J]. JOURNAL OF EXPERIMENTAL BOTANY, 1981, 32 (126) : 79 - 91
  • [12] DuBois G.E., 2000, Encyclopedia of Food Science Technology, P2245
  • [13] EPSTEIN E, 1972, P412
  • [14] Pineapple (Ananas comosus L-Merr) micropropagation in temporary immersion systems
    Escalona, M
    Lorenzo, JC
    González, B
    Daquinta, M
    González, JL
    Desjardins, Y
    Borroto, CG
    [J]. PLANT CELL REPORTS, 1999, 18 (09) : 743 - 748
  • [15] Temporary immersion systems in plant micropropagation
    Etienne, H
    Berthouly, M
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 2002, 69 (03) : 215 - 231
  • [16] Influence of exogenously applied paclobutrazol on some physiological traits and growth of Stevia rebaudiana under in vitro drought stress
    Hajihashemi, Shokoofeh
    Ehsanpour, Ali Akbar
    [J]. BIOLOGIA, 2013, 68 (03) : 414 - 420
  • [17] Hossain M. A., 2008, International Journal of Sustainable Crop Production, V3, P1
  • [18] Nutrient factors affecting in vitro cultivation of Stevia rebaudiana
    Ibrahim I.A.
    Nasr M.I.
    Mohammedm B.R.
    El-Zefzafi M.M.
    [J]. Sugar Tech, 2008, 10 (3) : 248 - 253
  • [19] Plant growth regulators affecting in vitro cultivation of Stevia rebaudiana
    Ibrahim I.A.
    Nasr M.I.
    Mohammed B.R.
    El-Zefzafi M.M.
    [J]. Sugar Tech, 2008, 10 (3) : 254 - 259
  • [20] Nitrogen source, concentration, and NH4 +:NO3 - ratio influence shoot regeneration and hyperhydricity in tissue cultured Aloe polyphylla
    Ivanova, Mariyana
    Van Staden, Johannes
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 2009, 99 (02) : 167 - 174