Host Defense Peptide-Mimicking Poly(2-oxazoline)s Displaying Potent Activities toward Phytopathogens to Alleviate Antimicrobial Resistance in Agriculture

Given the limited types of agricultural bactericides and the rapid emergence of antimicrobial resistance, bacterial plant diseases pose a serious threat to agricultural production, which calls for effective antimicrobial agents with a low propensity for resistance. Host defense peptides (HDPs) have drawn significant attention for their broad-spectrum antimicrobial activity. In this study, we found that HPD-mimicking poly(2-oxazoline) Gly-POX20 exhibits potent activity against bacterial phytopathogens, with superior antibacterial selectivity and proteolytic stability compared to the natural HDP melittin. Compared to commonly used agricultural bactericides, Gly-POX20 displays more efficient antibiofilm activity and a lower propensity for resistance than does the antibiotic streptomycin, likely due to its antibacterial mechanism, which involves DNA interaction and generating lethal doses of ROS. In vivo studies reveal that Gly-POX20 is effective in preventing and treating phytopathogens without observable damage to plant tissues, suggesting that poly(2-oxazoline) could be a promising bactericide for agricultural applications.