The Pathogenic Role of Expanded CD8+CD28null Angiogenic T Cells in ANCA-Associated Vasculitis

被引：0

作者：

Shen, Haomiao ^{[1
,2
]}

Miao, Jinlin ^{[1
,2
]}

Sun, Haoyang ^{[1
,2
]}

Zhang, Kui ^{[1
,2
]}

Liu, Renli ^{[1
,2
]}

Li, Zichao ^{[3
]}

Zhang, Leyang ^{[3
]}

Zhang, Peiyan ^{[1
,2
]}

Wang, Jiawei ^{[1
,2
]}

Zhang, Bei ^{[1
,2
]}

Chen, Longyu ^{[1
,2
]}

Zheng, Zhaohui ^{[1
,2
]}

Zhu, Ping ^{[1
,2
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Clin Immunol, Xian 710032, Peoples R China

[2] Fourth Mil Med Univ, Natl Translat Sci Ctr Mol Med, Dept Cell Biol, Xian 710032, Peoples R China

[3] Fourth Mil Med Univ, Xijing Hosp, Dept Plast Surg, Xian 710032, Peoples R China

来源：

BIOMEDICINES | 2025年 / 13卷 / 01期

基金：

中国国家自然科学基金;

关键词：

angiogenic T cells; CD28(null); pathogenesis; endothelial dysfunction; CD28;

D O I：

10.3390/biomedicines13010026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Objectives: Angiogenic T cells (Tang) are crucial in promoting angiogenesis, with the loss of CD28 serving as a marker for highly differentiated and senescent T cells. This study aims to investigate the characteristics and potential roles of CD8(+)CD28(null) Tang in patients with ANCA-associated vasculitis (AAV). Methods: A cohort of AAV patients and matched healthy controls (HCs) were analyzed. Flow cytometry was used to assess the profiles of circulating CD8(+)CD28(null) Tang. In vitro functional assays were performed to evaluate the pathogenic properties of CD8(+)CD28(null) Tang. Results: CD8(+)CD28(null) Tang levels were significantly higher in the peripheral blood of AAV patients compared to HCs, and their levels were further increased in AAV patients with MPO+, p-ANCA(+), or interstitial lung disease compared to their respective control groups. Additionally, there was a positive correlation between both the percentage and absolute count of CD8(+)CD28(null) Tang and the Birmingham Vasculitis Activity Score (BVAS). In patients with a good treatment response, both the percentage and absolute count of CD8(+)CD28(null) Tang were significantly reduced, and this reduction was positively correlated with the decrease in BVAS scores. In vitro studies revealed that CD8(+)CD28(null) Tang displayed enhanced chemotactic properties, induced apoptosis in human umbilical vein endothelial cells (HUVECs), and inhibited their proliferation, migration, and tube formation. Conclusions: AAV patients exhibit increased levels of circulating CD8(+)CD28(null) Tang, which can be reduced following effective treatment. Furthermore, CD8(+)CD28(null) Tang may contribute to the pathogenesis of AAV by promoting apoptosis and inhibiting the proliferation, migration, and tube formation of HUVECs.

引用

页数：17