Natural Variability of Genomic Sequences of Mal d 1 Allergen in Apples as Revealed by Restriction Profiles and Homolog Polymorphism

被引：0

作者：

Urbanova, Lucia ^{[1
]}

Bilcikova, Jana ^{[1
]}

Moravcikova, Dagmar ^{[2
]}

Ziarovska, Jana ^{[2
]}

机构：

[1] Slovak Univ Agr, Res Ctr AgroBioTech, Tr A Hlinku 2, Nitra 94976, Slovakia

[2] Slovak Univ Agr, Inst Plant & Environm Sci, Fac Agrobiol & Food Resources, Agr Nitra, Tr A Hlinku 2, Nitra 94976, Slovakia

来源：

AGRONOMY-BASEL | 2024年 / 14卷 / 09期

关键词：

Bet v 1; Mal d 1; polymorphism; Malus domestica Borkh; BET V 1; BIRCH POLLEN; CROSS-REACTIVITY; MAJOR ALLERGEN; EPITOPE; FRAGMENT; ANTIBODY; CLONING;

D O I：

10.3390/agronomy14092056

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Apples are a popular fruit worldwide, with many health and nutritional benefits. However, this fruit is also among those that, particularly in Central and Northern Europe, are allergenic due to the Mal d 1 allergen. Mal d 1 is a homologous allergen to Bet v 1-the main pollen allergen of birch. In this study, two different approaches were used to identify the natural length polymorphism of Bet v 1 homologs in apple varieties, with the aim of characterizing their effectiveness. BBAP (Bet v 1 based amplified polymorphism) and RFLP (restriction fragments length polymorphism) profiles were characterized and compared. RFLP analysis recognizes the genetic diversity of M. domestica Mal d 1 sequences at a relatively low level. In BBAP profiles, the genetic dissimilarity was up to 50%, which appears suitable for intraspecific fingerprinting and serves as an additional method for RFLP analysis. RFLP analysis was able to distinguish some varieties that BBAP could not, such as Sonet.

引用

页数：13

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