The presence of yeasts and bacteria in free-living amoebae isolated from COVID-19 patients: concern for secondary infections

被引：0

作者：

Mirabedini, Zahra ^{[1
]}

Niyyati, Maryam ^{[2
,3
]}

Rahimi, Hanieh Mohammad ^{[4
]}

Jevinani, Sara Soleimani ^{[4
]}

Fatemi, Marziye ^{[2
]}

Tanhaei, Mohammad ^{[5
]}

Mohebbi, Seyed Reza ^{[5
]}

Yadegar, Abbas ^{[4
]}

Abolghasemi, Sara ^{[3
]}

Mazar, Zahra Arab ^{[1
]}

Mirjalali, Hamed ^{[4
]}

机构：

[1] Univ Tehran Med Sci, Sch Publ Hlth, Dept Med Parasitol & Mycol, Tehran, Iran

[2] Shahid Beheshti Univ Med Sci, Fac Med, Dept Med Parasitol & Mycol, Tehran, Iran

[3] Shahid Beheshti Univ Med Sci, Infect Dis & Trop Med Res Ctr, Tehran, Iran

[4] Shahid Beheshti Univ Med Sci, Res Inst Gastroenterol & Liver Dis, Foodborne & Waterborne Dis Res Ctr, Tehran, Iran

[5] Shahid Beheshti Univ Med Sci, Res Inst Gastroenterol & Liver Dis, Gastroenterol & Liver Dis Res Ctr, Tehran, Iran

来源：

INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH | 2024年

关键词：

COVID-19; FLA; endosymbionts; yeasts; bacteria; ACANTHAMOEBA; IDENTIFICATION; PCR; TRANSMISSION; GENUS;

D O I：

10.1080/09603123.2024.2409830

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study aimed to investigate the presence of SARS-CoV-2, yeasts, and bacteria in isolated free-living amoeba (FLA) from COVID-19 patients. Nasopharyngeal swabs (n = 60) were obtained from COVID-19 patients. After cultivation, morphological characterization, and RNA/DNA extraction, the presence of selected microorganisms was investigated. From 60 COVID-19 samples, 18 (30%) were positive for FLA. Acanthamoeba sp. Naegleria australiensis, Tetramitus sp. and Vermamoeba vermiformis were characterized in 12 (80%), 1 (6.66%), 2 (13.33%), and 7 (38.88%) of samples, respectively. SARS-CoV-2 RNA was not detected in FLA. Candida albicans, C. tropicalis, and C. parapsilosis were detected in (11/18; 61.11%), (3/18; 16.67%), and (3/18; 16.67%) of samples, respectively. Geotrichum candidum was detected in 10/18 (55.55%) of samples. Streptococcus spp. and Staphylococcus spp. were identified in 16/18 (88.88%) and 3/18 (16.67%), respectively. The presence of yeasts and bacteria signifies the possible role of FLA in distribution of secondary infections in susceptible patients.

引用

页码：1846 / 1859

页数：14

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