Immunogenicity and Protective Efficacy of Baculovirus-Expressed SARS-CoV-2 Envelope Protein in Mice as a Universal Vaccine Candidate

被引：0

作者：

Oguzoglu, Tuba cigdem ^{[1
]}

Hanifehnezhad, Alireza ^{[1
]}

Khabbazi, Saber Delpasand ^{[2
]}

Karayel-Hacioglu, Ilke ^{[1
]}

Kaynarcalidan, Onur ^{[3
]}

Firat, Zehra ^{[4
]}

Filazi, Nazlican ^{[5
,6
]}

Erdem-Sahinkesen, Eda ^{[5
,7
]}

Gul, Buket ^{[5
]}

Karabulut, Muhammed Cesim ^{[1
,5
]}

Koba, Enes ^{[5
]}

Adiguzel, Ece ^{[8
]}

Senlik, Elif Irem ^{[5
]}

Korkulu, Emrah ^{[5
,9
]}

Demirden, Cansu ^{[5
,10
]}

Sahinkesen, Ilker ^{[11
]}

Ceylan, Ahmet ^{[12
]}

Muratoglu, Hacer ^{[13
]}

Vural, Sevil ^{[14
]}

Demirbag, Zihni ^{[15
]}

Ozkul, Aykut ^{[1
]}

机构：

[1] Ankara Univ, Fac Vet Med, Dept Virol, TR-06070 Ankara, Turkiye

[2] Yozgat Bozok Univ, Inst Hemp Res, Dept Agr & Food, TR-66900 Yozgat, Turkiye

[3] Heinrich Heine Univ, Dusseldorf Univ Hosp, Inst Virol, D-40225 Dusseldorf, Germany

[4] Ankara Univ, Biotechnol Inst, Ankara, Turkiye

[5] Ankara Univ, Grad Sch Hlth Sci, TR-06560 Ankara, Turkiye

[6] Hatay Mustafa Kemal Univ, Fac Vet Med, Dept Virol, TR-31040 Hatay, Turkiye

[7] Hacettepe Univ, Vaccine Inst, Dept Vaccine Technol, TR-06100 Ankara, Turkiye

[8] Republ Turkiye Minist Agr & Forestry, Atkaracalar Dist Directorate, TR-18310 Cankiri, Turkiye

[9] Kafkas Univ, Fac Vet Med, Dept Virol, TR-36000 Kars, Turkiye

[10] Republ Turkiye Minist Agr & Forestry, East Anatolian Agr Res Inst, Erzurum, Turkiye

[11] Diagen Biotechnol Syst Hlth Serv & Automat Ind, TR-06070 Ankara, Turkiye

[12] Ankara Univ, Fac Vet Med, Dept Histol & Embryol, TR-06070 Ankara, Turkiye

[13] Karadeniz Tech Univ, Fac Sci, Dept Mol Biol & Genet, TR-61080 Trabzon, Turkiye

[14] Ankara Univ, Fac Vet Med, Dept Pathol, TR-06070 Ankara, Turkiye

[15] Karadeniz Tech Univ, Fac Sci, Dept Biol, TR-61080 Trabzon, Turkiye

来源：

VACCINES | 2024年 / 12卷 / 09期

关键词：

SARS-CoV-2; envelope protein; recombinant; baculovirus; STRUCTURAL PROTEINS; INTERLEUKIN-10; CORONAVIRUS; IL-2;

D O I：

10.3390/vaccines12090977

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The envelope (env) protein of SARS-CoV-2, a pivotal component of the viral architecture, plays a multifaceted role in viral assembly, replication, pathogenesis, and ion channel activity. These features make it a significant target for understanding virus-host interactions and developing vaccines to combat COVID-19. Recent structural studies provide valuable insights into the conformational dynamics and membrane topology of the SARS-CoV-2 env protein, shedding light on its functional mechanisms. The strong homology and highly conserved structure of the SARS-CoV-2 env protein shape its immunogenicity and functional characteristics. This study examines the ability of the recombinant SARS-CoV-2 env protein to stimulate an immune response. In this study, recombinant envelope proteins were produced using the baculovirus expression system, and their potential efficacy was evaluated in both in vivo and in vitro models. Our results reveal that the env protein of SARS-CoV-2 stimulates humoral and cellular responses and highlight its potential as a promising vaccine candidate for combating the ongoing pandemic.

引用

页数：17

共 40 条

[1] In silico analysis of the substitution mutations and evolutionary trends of the SARS-CoV-2 structural proteins in Asia [J].

Abavisani, Mohammad ;

Rahimian, Karim ;

Kodori, Mansoor ;

Khayami, Reza ;

Sisakht, Mahsa Mollapour ;

Mahmanzar, Mohammadamin ;

Meshkat, Zahra .

IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 2022, 25 (11) :1299-1307

[2] Global landscape of SARS-CoV-2 mutations and conserved regions [J].

Abbasian, Mohammad Hadi ;

Mahmanzar, Mohammadamin ;

Rahimian, Karim ;

Mahdavi, Bahar ;

Tokhanbigli, Samaneh ;

Moradi, Bahman ;

Sisakht, Mahsa Mollapour ;

Deng, Youping .

JOURNAL OF TRANSLATIONAL MEDICINE, 2023, 21 (01)

[3] Preliminary Identification of Potential Vaccine Targets for the COVID-19 Coronavirus (SARS-CoV-2) Based on SARS-CoV Immunological Studies [J].

Ahmed, Syed Faraz ;

Quadeer, Ahmed A. ;

McKay, Matthew R. .

VIRUSES-BASEL, 2020, 12 (03)

[4] Development of new vaccine target against SARS-CoV2 using envelope (E) protein: An evolutionary, molecular modeling and docking based study [J].

Bhattacharya, Shreya ;

Banerjee, Arundhati ;

Ray, Sujay .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2021, 172 :74-81

[5]

Bode C, 2011, EXPERT REV VACCINES, V10, P499, DOI [10.1586/ERV.10.174, 10.1586/erv.10.174]

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7] Contributions of the structural proteins of severe acute respiratory syndrome coronavirus to protective immunity [J].

Buchholz, UJ ;

Bukreyev, A ;

Yang, LJ ;

Lamirande, EW ;

Murphy, BR ;

Subbarao, K ;

Collins, PL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (26) :9804-9809

[8] Characterization of the SARS-CoV-2 E Protein: Sequence, Structure, Viroporin, and Inhibitors [J].

Cao, Yipeng ;

Yang, Rui ;

Lee, Imshik ;

Zhang, Wenwen ;

Sun, Jiana ;

Wang, Wei ;

Meng, Xiangfei .

PROTEIN SCIENCE, 2021, 30 (06) :1114-1130

[9] Associations between Serum Interleukins (IL-1β, IL-2, IL-4, IL-6, IL-8, and IL-10) and Disease Severity of COVID-19: A Systematic Review and Meta-Analysis [J].

Chang, Yuanmin ;

Bai, Mengru ;

You, Qinghai .

BIOMED RESEARCH INTERNATIONAL, 2022, 2022

[10] Broadly neutralizing antibodies to SARS-CoV-2 and other human coronaviruses [J].

Chen, Yanjia ;

Zhao, Xiaoyu ;

Zhou, Hao ;

Zhu, Huanzhang ;

Jiang, Shibo ;

Wang, Pengfei .

NATURE REVIEWS IMMUNOLOGY, 2023, 23 (03) :189-199

← 1 2 3 4 →