Antibiotic resistance genes in anaerobic digestion: Unresolved challenges and potential solutions

被引：1
作者：
Wang, Chunxiao [1 ]
Yin, Xiaole [1 ]
Xu, Xiaoqing [1 ]
Wang, Dou [1 ]
Wang, Yubo [1 ]
Zhang, Tong [1 ,2 ,3 ,4 ]
机构：
[1] Univ Hong Kong, Ctr Environm Engn Res, Dept Civil Engn, Environm Microbiome Engn & Biotechnol Lab,Pok Fu L, Hong Kong, Peoples R China
[2] Univ Hong Kong, Sch Publ Hlth, Hong Kong, Peoples R China
[3] Macau Univ Sci & Technol, Macau Inst Appl Res Med & Hlth, Macau, Peoples R China
[4] City Univ Hong Kong, State Key Lab Marine Pollut, Hong Kong, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2025年 / 419卷
基金：
中国国家自然科学基金;
关键词：
Anaerobic digestion (AD); Antimicrobial resistance (AMR); Antibiotics resistance gene (ARG); Antibiotic-resistant bacteria (ARB); Horizontal gene transfer (HGT); Mobile genetic elements (MGEs); CLASS; 1; INTEGRONS; MICROBIAL COMMUNITY; GRAPHENE OXIDE; REMOVAL; FATE; DYNAMICS; MANURE; ABUNDANCES; REDUCTION; BACTERIA;
D O I：
10.1016/j.biortech.2025.132075
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Antimicrobial resistance (AMR) threatens public health, necessitating urgent efforts to mitigate the global impact of antibiotic resistance genes (ARGs). Anaerobic digestion (AD), known for volatile solid reduction and energy generation, also presents a feasible approach for the removal of ARGs. This review encapsulates the existing understanding of ARGs and antibiotic-resistant bacteria (ARB) during the AD process, highlighting unresolved challenges pertaining to their detection and quantification. The questions raised and discussed include: Do current ARGs detection methods meet qualitative and quantitative requirements? How can we conduct risk assessments of ARGs? What happens to ARGs when they come into co-exposure with other emerging pollutants? How can the application of internal standards bolster the reliability of the AD resistome study? What are the potential future research directions that could enhance ARG elimination? Investigating these subjects will assist in shaping more efficient management strategies that employ AD for effective ARG control.
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页数：12
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共 137 条
  • [1] Agga G.E., Kasumba J., Loughrin J.H., Conte E.D., Anaerobic digestion of tetracycline spiked livestock manure and poultry litter increased the abundances of antibiotic and heavy metal resistance genes, Front. Microbiol., 11, (2020)
  • [2] Alimi O.S., Farner Budarz J., Hernandez L.M., Tufenkji N., Microplastics and nanoplastics in aquatic environments: aggregation, deposition, and enhanced contaminant transport, Environ. Sci. Tech., 52, 4, pp. 1704-1724, (2018)
  • [3] An X.-L., Abass O.K., Zhao C.-X., Xu M.-R., Pan T., Pu Q., Liao H., Li H., Zhu Y.-G., Su J.-Q., Nanopore sequencing analysis of integron gene cassettes in sewages and soils, Sci. Total Environ., 817, (2022)
  • [4] Arango-Argoty G., Garner E., Pruden A., Heath L.S., Vikesland P., Zhang L., DeepARG: a deep learning approach for predicting antibiotic resistance genes from metagenomic data, Microbiome, 6, pp. 1-15, (2018)
  • [5] Bai Y., Xu R., Wang Q.-P., Zhang Y.-R., Yang Z.-H., Sludge anaerobic digestion with high concentrations of tetracyclines and sulfonamides: Dynamics of microbial communities and change of antibiotic resistance genes, Bioresour. Technol., 276, pp. 51-59, (2019)
  • [6] Baker-Austin C., Wright M.S., Stepanauskas R., McArthur J., Co-selection of antibiotic and metal resistance, Trends Microbiol., 14, 4, pp. 176-182, (2006)
  • [7] Baym M., Stone L.K., Kishony R., Multidrug evolutionary strategies to reverse antibiotic resistance, Science, 351, 6268, (2016)
  • [8] Bickhart D.M., Watson M., Koren S., Panke-Buisse K., Cersosimo L.M., Press M.O., Van Tassell C.P., Van Kessel J.A.S., Haley B.J., Kim S.W., Assignment of virus and antimicrobial resistance genes to microbial hosts in a complex microbial community by combined long-read assembly and proximity ligation, Genome Biol., 20, pp. 1-18, (2019)
  • [9] Borchardt M.A., Boehm A.B., Salit M., Spencer S.K., Wigginton K.R., Noble R.T., The environmental microbiology minimum information (EMMI) guidelines: qPCR and dPCR quality and reporting for environmental microbiology, Environ. Sci. Tech., 55, 15, pp. 10210-10223, (2021)
  • [10] Buerge I.J., Keller M., Buser H.-R., Muller M.D., Poiger T., Saccharin and other artificial sweeteners in soils: estimated inputs from agriculture and households, degradation, and leaching to groundwater, Environ. Sci. Tech., 45, 2, pp. 615-621, (2011)
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