Heterologous Expression of Type II PKS Gene Cluster Leads to Diversified Angucyclines in Streptomyces albus J1074

被引：2

作者：

Zhang, Xiaoting ^{[1
]}

Zhang, Falei ^{[1
]}

Li, Chen ^{[1
]}

Li, Jiayi ^{[1
]}

Xu, Xiao ^{[1
]}

Zhu, Tianjiao ^{[1
]}

Che, Qian ^{[1
]}

Li, Deihai ^{[1
,2
,3
]}

Zhang, Guojian ^{[1
,2
,4
]}

机构：

[1] Ocean Univ China, Sch Med & Pharm, Key Lab Marine Drugs, Chinese Minist Educ, Qingdao 266003, Peoples R China

[2] Qingdao Marine Sci & Technol Ctr, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China

[3] Ocean Univ China, Sanya Oceanog Inst, Sanya 572025, Peoples R China

[4] Marine Biomed Res Inst Qingdao, Qingdao 266101, Peoples R China

来源：

MARINE DRUGS | 2024年 / 22卷 / 11期

基金：

中国国家自然科学基金;

关键词：

heterologous expression; II PKS; marine Streptomyces; angucycline; INTERGENERIC CONJUGATION; NATURAL-PRODUCTS; BIOSYNTHESIS; ANTIBIOTICS; METABOLITES; MODE;

D O I：

10.3390/md22110480

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Heterologous expression has emerged as an effective strategy in activating Streptomyces cryptic gene clusters or improving yield. Eight compounds were successfully obtained by heterologous expression of the type II PKS gene cluster spi derived from marine Streptomyces sp. HDN155000 in the chassis host Streptomyces albus J1074. The structures with absolute configurations were elucidated using extensive MS and NMR spectroscopic methods, as well as theoretical NMR calculations and electronic circular dichroism (ECD) calculations. Interestingly, compound WS009 Z (2) contains a rare thiomethyl group, angumycinone T (4) has a novel oxo-bridge formed between C12a and C4, and angumycinone X (3) showed cytotoxicity toward K562 and NCI-H446/EP cell lines.

引用

页数：13

共 45 条

[1] Gephyromycin, the first bridged angucyclinone, from Streptomyces griseus strain NTK 14 [J].