New and efficient AS-PCR molecular marker for selection of coffee resistant to coffee leaf rust

被引：0

作者：

de Almeida, Denia Pires ^{[1
]}

Carneiro, Deisy Guimaraes ^{[1
]}

Caixeta, Eveline Teixeira ^{[1
,2
]}

Alves, Danubia Rodrigues ^{[1
]}

Zambolim, Laercio ^{[1
]}

机构：

[1] Univ Fed Vicosa, Inst Biotecnol Aplicada Agr, Vila Matoso 205, BR-36570000 Vicosa, MG, Brazil

[2] Empresa Brasileira Pesquisa Agr Embrapa, PqEB, Ave W3 Norte Final S-N, BR-70770901 Brasilia, DF, Brazil

来源：

CROP BREEDING AND APPLIED BIOTECHNOLOGY | 2024年 / 24卷 / 04期

关键词：

Coffee breeding; sustainable coffee; marker-assisted selection (MAS); QTL; Hemileia vastatrix; HEMILEIA-VASTATRIX; SOFTWARE;

D O I：

10.1590/1984-70332024v24n4a47

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Coffee leaf rust, caused by Hemileia vastatrix, is the most important disease affecting Coffea arabica. The use of molecular markers through marker-assisted selection (MAS) is an effective approach to develop cultivars with multiple and durable resistance, which is economically and environmentally beneficial for this disease control. In this work, a new molecular marker based on allele-specific polymerase chain reaction (AS-PCR) associated with the quantitative trait locus (QTL) of the linkage group 5 (LG5), which confers resistance to races I, II and pathotype 001 of H. vastatrix, was developed. The CaRHv10-AS marker was validated in a segregating genetic mapping population of 247 F2 genotypes and demonstrated selection efficiency greater than 97% for genotypes with resistance to three races. Thus, this work provides a fast, robust, and affordable molecular marker for use in MAS, facilitating the development of coffee plants with multiple resistance and ensuring sustainable coffee production.

引用

页数：9

共 38 条

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