Isolation, characterization, and genome sequencing analysis of a novel phage HBW-1 of Salmonella

被引：2
作者：
Jiang, Zenghai [1 ]
Yaqoob, Muhammad Umar [2 ,3 ]
Xu, Yaohui [1 ]
Siddique, Abubakar [2 ]
Lin, Shuqi [1 ]
Hu, Siyu [1 ]
Ed-Dra, Abdelaziz [4 ]
Yue, Min [2 ,5 ,6 ]
机构：
[1] Henan Univ Anim Husb & Econ, Coll Vet Med, Zhengzhou 450046, Peoples R China
[2] Zhejiang Univ, Lab Mol Microbiol & Food Safety, Coll Anim Sci, Hangzhou 310058, Peoples R China
[3] Hangzhou King Techina Feed Co Ltd, Prov Key Agr Enterprise Res Inst King Techina, Hangzhou 311107, Peoples R China
[4] Sultan Moulay Slimane Univ, Higher Sch Technol, Lab Engn & Appl Technol, Mghila Campus,BP 591, Beni Mellal 23000, Morocco
[5] Zhejiang Univ, Hainan Inst, Sanya 572025, Peoples R China
[6] Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Life Sci, Key Lab Syst Hlth Sci Zhejiang Prov, Hangzhou 310024, Peoples R China
来源：
MICROBIAL PATHOGENESIS | 2025年 / 200卷
关键词：
Antimicrobial resistance; Phage therapy; Salmonella; Pullorum disease; Food control; Genome sequencing; BACTERIOPHAGE; ENTERICA; FOOD;
D O I：
10.1016/j.micpath.2025.107327
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Salmonella presents a significant threat to the health of animals and humans, especially with the rise of strains resistant to multiple drugs. This highlights the necessity for creating sustainable and efficient practical approaches to managing salmonellosis. The most recent and safest approach to combat antimicrobial resistanceassociated infections is lytic bacteriophages. This study recovered a Salmonella-specific phage HBW-1 from sewage and faecal samples from commercial chicken farms in Henan, China. Transmission electron microscopy showed that the phage possesses a polyhedral head and a tailed structure characteristic of bacteriophages. The phage HBW-1 exhibited favorable stability when subjected to elevated temperatures between 30 degrees C and 60 degrees C and pH levels between 3 and 12 for 1 h. The phage genome consists of double-stranded, circular DNA with a size of 43,095 bp and a GC content of 49.54 %. Notably, phage HBW-1 contains 62 genes encoding proteins and does not contain virulence or resistance genes commonly found in bacteria. Phage spectrum analysis indicates that the phage HBW-1 is strictly a lytic, exhibiting antibacterial activity against Salmonella Pullorum (100 %, n = 11), Salmonella Typhimurium (92.86 %, n = 42) and Salmonella Enteritidis (58.97 %, n = 39). Therefore, this study suggests that phage HBW-1 holds promise as a potential alternative for prevention and control of Pullorum Disease.
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页数：7
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