Chemistry-driven mass spectrometry for structural lipidomics at the C = C bond isomer level

As more and more studies have shown that lipid molecules play an important role in the whole biology, in-depth analysis of lipid structure has become particularly important in lipidomics. Mass spectrometry (MS), as the preferred tool for lipid analysis, has greatly promoted the development of this field. However, the existing MS methods still face many difficulties in the in-depth or even comprehensive analysis of lipid structure. In this review, we discuss recent advances in MS methods based on double bond-specific chemistries for the resolving of C = C location and geometry isomers of lipids. This progress has greatly advanced the lipidomics analysis to a deeper structural level and facilitated the development of structural lipid biology. (c) 2024 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.