Establishment and biological characterization of radioresistant colorectal cancer cell lines

被引:0
作者
Qu, Tian-Yin [1 ,2 ]
Dai, Qing [1 ,2 ]
Leng, Jing [1 ,2 ]
Fang, Lin [1 ,2 ]
Ma, Jing-Jing [3 ]
Chen, Rui [1 ,2 ]
Chen, Che [1 ,2 ]
Ran, Peng-Fei [1 ,2 ]
Zhou, Wen-Wen [1 ,2 ]
Liu, Chang [1 ,2 ]
Yu, Huang-Fei [1 ,2 ]
机构
[1] Zunyi Med Univ, Peoples Hosp ZunYi 1, Affiliated Hosp 3, Dept Oncol, 98 Fenghuang Rd, Zunyi 563000, Guizhou, Peoples R China
[2] Zunyi Med Univ, Peoples Hosp ZunYi 1, The Affiliated Hosp 3, Canc Dis Res Inst, Zunyi, Peoples R China
[3] Zunyi Med Univ, Affiliated Hosp 3, Peoples Hosp Zunyi 1, Dept Clin Lab, Zunyi, Peoples R China
来源
FEBS OPEN BIO | 2025年
基金
中国国家自然科学基金;
关键词
antioxidant; colorectal cancer; DNA damage repair; radioresistant; RADIOTHERAPY;
D O I
10.1002/2211-5463.70016
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Radiotherapy resistance is a major cause of recurrence and metastasis in colorectal cancer (CRC). We established radiotherapy-resistant cell lines to explore the molecular mechanisms of radiotherapy resistance in CRC. HT29 and HCT116 cells were subjected to repeated irradiation at 2 Gy to establish these lines. CCK-8 assay, colony formation, and xenograft tumor experiments were used to detect the radiosensitivity of the cells. DNA damage repair proteins, GSH content, and intracellular ROS were also assayed in parental and resistant cells. We successfully established HT29R and HCT116R radioresistant cell lines after fractionated irradiation, and the cells showed significant tolerance to further irradiation compared with the parental cells and a stronger capacity for DNA damage repair. Meanwhile, ionizing radiation significantly reduced GSH in HT29 and HCT116 parental cells but had no effect on GSH content in resistant cells. These results demonstrate that radioresistant colorectal cancer cell lines were successfully established by the method of continuous irradiation with 2 Gy. This provides a basis for further exploration of the mechanism of colorectal cancer radiotherapy resistance.
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页数:13
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