Optimization of extraction process of polysaccharide from Phylloporia fontanesiae and its simulated digestion in vitro

被引:1
作者
Ju, Hao [1 ,2 ]
Fang, Wei [3 ]
Li, Hai-Huang [2 ]
Fu, Ze [2 ]
Gong, Pi-Xian [2 ]
Liu, Yang [2 ]
Lu, Siqi [2 ]
Wu, Yan-Chao [2 ]
Li, Hui-Jing [1 ,2 ]
机构
[1] Harbin Inst Technol, Sch Chem & Chem Engn, Harbin 150006, Peoples R China
[2] Harbin Inst Technol, Weihai Marine Organism & Med Technol Res Inst, Weihai Key Lab Act Factor Marine Prod, Weihai, Peoples R China
[3] Shandong Univ, Weihai Municipal Hosp, Cheeloo Coll Med, Dept Pharm, Weihai, Peoples R China
基金
中国国家自然科学基金;
关键词
antioxidant; Box-Behnken design; Phylloporia fontanesiae polysaccharide; simulated gastrointestinal digestion; REDUCING ANTIOXIDANT POWER; IMMUNOMODULATORY ACTIVITY; PECTIN; PURIFICATION; ESTERIFICATION; SPECTROSCOPY; PROTEIN; ASSAY; MILK;
D O I
10.1111/1750-3841.17381
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
In this study, Phylloporia fontanesiae polysaccharide was successfully isolated through a sequential water extraction and alcohol precipitation process. Utilizing the Box-Behnken design, the extraction process was optimized based on single-factor experiments, considering variables such as the material-to-liquid ratio, extraction temperature, extraction time, and the number of extractions. The polysaccharide composition of P. fontanesiae is predominantly composed of mannose, glucuronic acid, glucose, and galactose, with a molar mass ratio of 4.31:4.10:36.83:1, along with minor amounts of aminoglucose and fucose. The polysaccharide fraction of P. fontanesiae comprises two distinct components, possessing relative molecular masses of 8.85 kDa and 134.03 kDa. Notably, the polysaccharide exhibited significant antioxidant activity. After undergoing simulated gastrointestinal digestion, no significant changes were observed in its antioxidant activity, molecular weight, or monosaccharide composition. This study not only enhanced the extraction efficiency of P. fontanesiae polysaccharide but also provided valuable insights into its composition, structure, and digestion characteristics. Practical ApplicationThe optimum extraction process, stability, and antioxidant activity of Phylloporia fontanesiae polysaccharide during simulated digestion of gastrointestinal tract were studied. The results provide a theoretical basis for the development and application of this polysaccharide in the field of food and health products.
引用
收藏
页码:8804 / 8818
页数:15
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