Cirsii Herba glycoprotein promotes macrophage M1 polarization through MAPK and NF-κB signaling pathways via interaction with TLR4

被引：1
作者：
Zhao, Ming [1 ]
Qin, Shiyue [1 ]
Wang, Jiaqi [1 ]
Zheng, Sichun [1 ]
Ma, Xiaodan [2 ]
Xu, Wei [1 ]
机构：
[1] Zhejiang Univ, Coll Anim Sci, Dept Vet Med, MOA Key Lab Anim Virol,Ctr Vet Sci, Hangzhou 310058, Peoples R China
[2] Univ Hong Kong, LKS Fac Med, Dept Microbiol, Pokfulam, Hong Kong, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2025年 / 296卷
基金：
中国国家自然科学基金;
关键词：
Cirsii Herba glycoprotein; Macrophage; Immunomodulation; TLR4; Signaling pathway; POLYSACCHARIDE; ACTIVATION;
D O I：
10.1016/j.ijbiomac.2025.139687
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The present study aimed to extract and purify the glycoprotein from Cirsii Herba (CHPs), and investigate its immunomodulatory activity and molecular mechanism in RAW264.7 macrophages. The results showed that CHPs contained 14.8% carbohydrates and 80.4% proteins. CHPs were identified as glycoprotein around 70 kDa and contained 17 different amino acids, in which the Glu and Asp were predominant. The carbohydrate chain in CHPs was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose with the molecular ratio of 6.387: 24.358: 5.766: 8.877: 12.098: 20.427: 7.090: 14.997. CHPs significantly boosted pinocytic and phagocytic activities, increased the secretions of inflammatory factors (NO, TNF-alpha and IL-6) and chemokines (CXCL2 and CXCL10), and promoted the expressions of accessory and costimulatory molecules (CD40, CD80, CD86, MHC I and MHC II). RNA-seq analysis identified 721 DEGs, 1575 GO terms and 69 KEGG pathways. The pathway inhibition assay presented that MAPK and NF-kappa B pathways were essential to macrophage activation by CHPs. TLR4 was revealed as a functional receptor and involved in the early recognition of CHPs. These results indicated that CHPs as a glycoprotein promoted macrophage polarization to M1 phenotype mainly via TLR4-dependent MAPK and NF-kappa B pathways.
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页数：11
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