Direct Cloning and Heterologous Expression of the Dmxorosin Biosynthetic Gene Cluster from Streptomyces thermolilacinus SPC6, a Halotolerant Actinomycete Isolated from the Desert in China

被引：0
作者：
Dong, Maoxing [1 ]
Feng, Huyuan [1 ]
Zhang, Wei [2 ]
Ding, Wei [3 ]
机构：
[1] Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China
[2] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Key Lab Extreme Environm Microbial Resources & Eng, Lanzhou 730000, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2025年 / 26卷 / 04期
基金：
中国科学院西部之光基金; 中国国家自然科学基金;
关键词：
cryptic gene cluster; dehydratase; heterologous expression; red/ET recombination; PEPTIDE NATURAL-PRODUCTS; LANTHIPEPTIDE; DISCOVERY; INSIGHTS; ANTIBIOTICS; COELICOLOR; ANTIFUNGAL; MECHANISM; EVOLUTION; BACTERIA;
D O I：
10.3390/ijms26041492
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Streptomyces thermolilacinus SPC6 is a halotolerant strain isolated from the Linze Desert in China. It has a very high growth rate and short life cycle compared to other Streptomycetes, including the model organism Streptomyces coelicolor. The one strain-many compounds fermentation approach and global natural products investigation revealed that Streptomyces thermolilacinus SPC6 exhibits impressive productivity of secondary metabolites. Genome mining uncovered 20 typical secondary metabolic biosynthetic gene clusters (BGC), with a BGC dmx identified as completely silent. Subsequently, this cryptic BGC was successfully directly cloned and heterologously expressed in Streptomyces hosts, resulting in the discovery of a new lanthipeptide, dmxorosin. Notably, the proposed biosynthetic pathway indicates its potential as a basis for the synthetic biology of new lanthipeptide.
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页数：16
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